RBRC04950

Information1

Image
BRC No.RBRC04950
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Traf6<tm1Jino>
Former Common nameB6-Traf6 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentA 2.5 kb genomic fragement was replaced with a neomycin selection cassette. The mutant mice were crossed with C57BL/6.
Strain descriptionTraf6 knockout mice. A 2.5 kb genomic fragment was replaced with a neomycin selection cassette. Homozygous mutant mice exhibit severe osteopetrosis and abnormal immune cell development and function, and die within around 2 weeks after birth. Homozygous mice represented only 12% of offspring. C57BL/6 background (RBRC04950), BALB/c background (RBRC05386).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesGenes Cells., 4, 353-362 (1999). 10421844

Health Report

Examination Date / Room / Rack2013/01/21Room:4-ARack:D
2012/12/19Room:4-ARack:D
2012/12/17Room:4-ARack:D
2012/11/26Room:4-ARack:D
2012/11/26Room:4-ARack:D
2012/09/24Room:4-ARack:D
2012/07/23Room:4-ARack:D
2012/05/21Room:4-ARack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Traf6TNF receptor-associated factor 62Traf6<tm1Jino>targeted mutation 1, Jun-ichiro Inoue

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse Polr2a promoter

Information2

Donor DNAmouse Polr2a promoter, E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Traf6 genomic DNA
Research application
Specific Term and Conditions1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
2. RECIPIENT must contact the DEPOSITOR in the case of application for any patents,any manuscripts, any public presentation,or commercial use, based on the results from the use of the BIOLOGICAL RESOURCE.
3. RECIPIENT must not yield the BIOLOGICAL RESOURCE to other parties or persons.
DepositorJunichiro Inoue (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Sep 2012

BRC mice in Publications

Ismail HM, Didangelos A, Vincent TL, Saklatvala J.
Rapid Activation of Transforming Growth Factor beta-Activated Kinase 1 in Chondrocytes by Phosphorylation and K(63) -Linked Polyubiquitination Upon Injury to Animal Articular Cartilage.
Arthritis & rheumatology (Hoboken, N.J.) 69(3) 565-575(2016) 27768832