Strain Data Sheet

RBRC04949

Information1

Image
BRC No.RBRC04949
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB(Cg)-Psmd14<tm1.1Smta>
Former Common nameB6-Psmd14/FlagGFP KI (Rpn11-Flag/GFP)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shigeo Murata, The Tokyo Metropolitan Institute of Medical Sicence in 2002.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesMethods Enzymol., 399, 227-240 (2005). 16338359

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Psmd14proteasome (prosome, macropain) 26S subunit, non-ATPase, 142Psmd14<tm1.1Smta>targeted mutation 1.1, Shigeo Murata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)2FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HisHis tag (synthetic)2His

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Information2

Donor DNAYeast FRT (flippase recombination target) site, Phage P1 loxP site, Aequorea victoria GFP, Flag tag, His tag
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe recipient must obtain a prior written consent on use of it from the depositor. In publishing the research results obtained by used of the biological resource, the recipient must describe the depositor as a coauthor of the study in the first publication only.
DepositorShigeo Murata (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data