Strain Data Sheet

RBRC04949

Strain Information

Image
BRC No.RBRC04949
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB(Cg)-Psmd14<tm1.1Smta>
Former Common nameB6-Psmd14/FlagGFP KI (Rpn11-Flag/GFP)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shigeo Murata, The Tokyo Metropolitan Institute of Medical Sicence in 2002.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Large- and small-scale purification of mammalian 26S proteasomes.
Hirano Y, Murata S, Tanaka K
Methods Enzymol., 399, 227-240 (2005). 16338359

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAGFLAG tag (synthetic)2FLAG
Frtyeast FRT (flippase recombination target) site2Frt
GFPGreen Fluorescent Protein (Aequorea victoria)2
HisHis tag (synthetic)2His
Psmd14proteasome (prosome, macropain) 26S subunit, non-ATPase, 142Psmd14targeted mutation 1.1, Shigeo Murata
loxPphage P1 loxP2loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAYeast FRT (flippase recombination target) site, Phage P1 loxP site, Aequorea victoria GFP, Flag tag, His tag
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by used of the biological resource, the recipient must describe the depositor as a coauthor of the study in the first publication only.
    DepositorShigeo Murata (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data