Strain Data Sheet

RBRC04948

Strain Information

Image
BRC No.RBRC04948
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB(Cg)-Psmg1<tm1.1Smta>
Former Common nameB6-Psmg1 Floxed (PAC1 cKO)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shigeo Murata, Guraduate School of Pharmaceutical Sciences, The University of Tokyo in 2010. A loxP was inserted unstream of exon 2 and an FRT falked neo cassette with a 3’ loxP site was inserted downstream of exon 2. Neo cassette was removed by Flp mediated recombination.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
PAC1 gene knockout reveals an essential role of chaperone-mediated 20S proteasome biogenesis and latent 20S proteasomes in cellular homeostasis.
Sasaki K, Hamazaki J, Koike M, Hirano Y, Komatsu M, Uchiyama Y, Tanaka K, Murata S
Mol. Cell. Biol., 30, 3864-3874 (2010). 20498273

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site16Frt
Psmg1proteasome (prosome, macropain) assembly chaperone 116Psmg1targeted mutation 1.1, Shigeo Murata
loxPphage P1 loxP16loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cerebellar granule cell precursor proliferation(MP:0009968)

  • abnormal cerebellum development(MP:0000854)

  • abnormal cerebellum morphology(MP:0000849)

  • abnormal cerebral hemisphere morphology(MP:0008540)

  • abnormal dentate gyrus morphology(MP:0000812)
    • more 10 phenotypes
  • abnormal hepatocyte morphology(MP:0000607)

  • abnormal hepatocyte physiology(MP:0010080)

  • ataxia(MP:0001393)

  • limb grasping(MP:0001513)

  • no abnormal phenotype detected(MP:0002169)

  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, complete penetrance(MP:0011085)

  • small hippocampus(MP:0008283)

  • thin cerebral cortex(MP:0006254)

  • tremors(MP:0000745)
    • Detailed phenotype data

    Ordering Information

    Donor DNAYeast FRT (flippase recombination target) site, Phage P1 loxP site, Mouse Psmg1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 30, 3864-3874 (2010).In publishing the research results obtained by used of the biological resource, the recipient must describe the depositor as a coauthor of the study in the first publication only.
    DepositorShigeo Murata (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data