Strain Data Sheet

RBRC04948

Information1

Image
BRC No.RBRC04948
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB(Cg)-Psmg1<tm1.1Smta>
Former Common nameB6-Psmg1 Floxed (PAC1 cKO)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shigeo Murata, Guraduate School of Pharmaceutical Sciences, The University of Tokyo in 2010. A loxP was inserted unstream of exon 2 and an FRT falked neo cassette with a 3’ loxP site was inserted downstream of exon 2. Neo cassette was removed by Flp mediated recombination.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesMol. Cell. Biol., 30, 3864-3874 (2010). 20498273

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Psmg1proteasome (prosome, macropain) assembly chaperone 116Psmg1<tm1.1Smta>targeted mutation 1.1, Shigeo Murata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Information2

Donor DNAMouse Psmg1 genomic DNA, Yeast FRT (flippase recombination target) site, Phage P1 loxP site
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 30, 3864-3874 (2010).In publishing the research results obtained by used of the biological resource, the recipient must describe the depositor as a coauthor of the study in the first publication only.
DepositorShigeo Murata (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data