Strain Data Sheet

RBRC04945

Strain Information

Image
BRC No.RBRC04945
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129S4(B6)-Il5<tm1Ktk>
Former Common nameBALB-IL5/Venus KI
H-2 Haplotype
ES Cell lineAK7 [129S4/SvJaeSor]
Background strainBALB/cCrSlc
Appearance
Strain developmentDeveloped by Kiyoshi Takatsu, Graduate School of Medicine and Pharmaceutical, University of Toyama / Toyama Prefectural Institute for Pharmaceutical Research / The Institute of Medical Science, The University of Tokyo in 2008.
Strain description
Colony maintenanceHeterozygote x Wild-type [BALB/cCrSlc]
References
Defective B-1 cell development and impaired immunity against Angiostrongylus cantonensis in IL-5R alpha-deficient mice.
Yoshida T, Ikuta K, Sugaya H, Maki K, Takagi M, Kanazawa H, Sunaga S, Kinashi T, Yoshimura K, Miyazaki J, Takaki S, Takatsu K
Immunity, 4, 483-494 (1996). 8630733

IL-5-deficient mice have a developmental defect in CD5+ B-1 cells and lack eosinophilia but have normal antibody and cytotoxic T cell responses.
Kopf M, Brombacher F, Hodgkin P D, Ramsay A J, Milbourne E A, Dai W J, Ovington K S, Behm C A, Kohler G, Young I G, Matthaei K I
Immunity, 4, 15-24 (1996). 8574848

Identification of innate IL-5-producing cells and their role in lung eosinophil regulation and antitumor immunity.
Ikutani M, Yanagibashi T, Ogasawara M, Tsuneyama K, Yamamoto S, Hattori Y, Kouro T, Itakura A, Nagai Y, Takaki S, Takatsu K
J. Immunol., 188(2):703-713 (2012). 22174445

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Il5interleukin 511Il5targeted mutation 1, Kiyoshi Takatsu
VenusYellow Fluorescent Protein (Aequorea victoria)11Venus
loxPphage P1 loxP11loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B-1 B cell morphology(MP:0004940)

  • decreased interleukin-5 secretion(MP:0008703)

  • increased metastatic potential(MP:0001272)
    • Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish Venus (a mutant of yellow fluorescdent ptotein, derived from green fluorescent protein), phage P1 loxP site, mouse IL-5 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 188(2):703-713 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and Life technologies and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR and Life technologies in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorKiyoshi Takatsu (University of Toyama)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data