RBRC04944

Information1

Image
BRC No.RBRC04944
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Il5<tm1.1Ktk>
Former Common nameB6-IL5/Venus KI
H-2 Haplotype
ES Cell lineAK7 [129S4/SvJaeSor]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kiyoshi Takatsu, Graduate School of Medicine and Pharmaceutical, University of Toyama / Toyama Prefectural Institute for Pharmaceutical Research / The Institute of Medical Science, The University of Tokyo in 2008.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesImmunity, 4, 483-494 (1996). 8630733
J. Immunol., 188(2):703-713 (2012). 22174445
Immunity, 4, 15-24 (1996). 8574848

Health Report

Examination Date / Room / Rack2012/09/24Room:4-ARack:B
2012/07/23Room:4-ARack:B
2012/05/21Room:4-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Il5interleukin 511Il5<tm1.1Ktk>targeted mutation 1.1, Kiyoshi Takatsu

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)11Venus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Information2

Donor DNAJellyfish Venus (a mutant of yellow fluorescdent ptotein, derived from green fluorescent protein) cDNA, phage P1 loxP site, mouse IL-5 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 188(2):703-713 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp).
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and Life tecnologies and must obtain a prior written consent from the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR and Life tecnologies in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKiyoshi Takatsu (University of Toyama)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Approval form Form V (English)

BRC mice in Publications

No Data