Strain Data Sheet

RBRC04942

Strain Information

Image
BRC No.RBRC04942
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Cntn2<tm1Fuka>
Former Common nameTAG-1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Kazutada Watanabe, Nagaoka University of Technology in 2001. The region between exon 2 and 5, encoding the translational start codon through the first Ig-like domain was replaced with a neo cassette.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Association of TAG-1 with Caspr2 is essential for the molecular organization of juxtaparanodal regions of myelinated fibers.
Traka M, Goutebroze L, Denisenko N, Bessa M, Nifli A, Havaki S, Iwakura Y, Fukamauchi F, Watanabe K, Soliven B, Girault J A, Karagogeos D
J. Cell Biol., 162, 1161-1172 (2003). 12975355

TAG-1-deficient mice have marked elevation of adenosine A1 receptors in the hippocampus.
Fukamauchi F, Aihara O, Wang Y J, Akasaka K, Takeda Y, Horie M, Kawano H, Sudo K, Asano M, Watanabe K, Iwakura Y
Biochem. Biophys. Res. Commun., 281, 220-226 (2001). 11178983

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cntn2contactin 21Cntn2targeted mutation 1, Fumihiko Fukamauchi
  • epilepsy, familial adult myoclonic, 5(MedGEN)
    • neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)
  • epilepsy, familial adult myoclonic, 5(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • increased susceptibility to pharmacologically induced seizures(MP:0002906)

  • nervous system phenotype(MP:0003631)

  • sporadic seizures(MP:0000951)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse TAG-1 (Cntn2) genomic DNA, E. coli Neomycin resistance gene
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 281, 220-226 (2001). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEVELOPER, Dr. Kazutada Watanabe is requested.
    DepositorYasushi Shimoda (Nagaoka University of Technology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data