Strain Data Sheet

RBRC04920

Strain Information

Image
BRC No.RBRC04920
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Il4<tm1Kopf>
Former Common nameB6.129S2-Il4<tm1Kopf>
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Manfred Kopf, Max-Planck-Institut fur Immunbiologie in 1993. The mice were backcrossed to C57BL/6 for at least 8 times by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo.
Strain descriptionIL-4 knockout mice. Homozygous mutant mice show increased susceptibility to parasitic infection.
Colony maintenance
References
Disruption of the murine IL-4 gene blocks Th2 cytokine responses.
Kopf M, Le Gros G, Bachmann M, Lamers M C, Bluethmann H, Kohler G
Nature, 362, 245-248 (1993). 8384701

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Il4interleukin 411Il4targeted mutation 1, Manfred Kopf
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal response to infection(MP:0005025)

  • decreased IgE level(MP:0002492)

  • decreased IgG1 level(MP:0008495)

  • decreased eosinophil cell number(MP:0005012)

  • decreased interleukin-10 secretion(MP:0008661)
    • more 5 phenotypes
  • decreased interleukin-3 secretion(MP:0008697)

  • decreased interleukin-4 secretion(MP:0008700)

  • decreased interleukin-5 secretion(MP:0008703)

  • increased IgG level(MP:0002493)

  • increased susceptibility to parasitic infection(MP:0005027)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Il4 genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 362(6417):245-248 (1993). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYoichiro Iwakura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data