Strain Data Sheet

RBRC04918

Information1

Image
BRC No.RBRC04918
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Il6<tm1Kopf>
Former Common nameB6.129S2-Il6<tm1Kopf>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Manfred Kopf, Max-Planck-Institut fur Immunbiologie in 1994. The mice were backcrossed to C57BL/6 for at least 8 times by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo.
Strain descriptionIL-6 knockout mice. Homozygous mutant mice show decreased immune competence. slight overweight with aging.
Colony maintenance
ReferencesNature, 368, 339-342 (1994). 8127368

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Il6interleukin 65Il6<tm1Kopf>targeted mutation 1, Manfred Kopf

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA5

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), Neomycin resistance gene (E. coli), PolyA signal (SV40), mouse Il6 genomic DNA
Research applicationImmunology and Inflammation Research
covid19_onset
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 368, 339-342 (1994).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYoichiro Iwakura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data