Strain Data Sheet

RBRC04916

Information1

Image
BRC No.RBRC04916
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gnb1<Gt(prvSStrap)4B8Yiw>
Former Common nameB6-Gnb1 Gt#4B8
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2010. The mice were backcrossed to C57BL/6 for at least 6 times.
Strain descriptionGbeta1 knockout mice. Homozygous mutant mice are embryonic lethal.
Colony maintenance
ReferencesDev. Dyn., 239, 1089-1101 (2010). 20186915

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gnb1guanine nucleotide binding protein (G protein), beta 14Gnb1<Gt(prvSStrap)4B8Yiw>gene trap 4B8, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CD4CD4 TM domain sequence (rat)4CD4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hyghygromycin phasphotransferase (E. coli)4Hyg

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
LTRLTR (Mo-MuLV, Mo-MuSV)4LTR

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SAimmunoglobulin splice acceptor (mouse)4SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
polyATK polyA signal (HSV)4polyA

Information2

Donor DNArabbit beta-globin intron, mouse immunoglobulin splice acceptor (SA), rat CD4 TM domain sequence, E. coli lacZ gene, E. coli hygromycin resistance gene, HSV TK polyA signal, Mo-MuLV, Mo-MuSV LTR
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Dyn., 239, 1089-1101 (2010).Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use.
DepositorYoichiro Iwakura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data