Strain Data Sheet

RBRC04911

Strain Information

Image
BRC No.RBRC04911
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Mc2r<tm1Dchi>
Former Common nameB6.129P2-Mc2r<tm1Dchi>
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo and Mitsumasa Kubo, Hokkaido University of Education in 2007. The mice were backcrossed to C57BL/6 for at least 5 times.
Strain descriptionMC2R knockout mice. Most of homozygous mutant mice were perinatal lethal.
Colony maintenance
References
Melanocortin 2 receptor is required for adrenal gland development, steroidogenesis, and neonatal gluconeogenesis.
Chida D, Nakagawa S, Nagai S, Sagara H, Katsumata H, Imaki T, Suzuki H, Mitani F, Ogishima T, Shimizu C, Kotaki H, Kakuta S, Sudo K, Koike T, Kubo M, Iwakura Y
Proc. Natl. Acad. Sci. USA, 104, 18205-18210 (2007). 17989225

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mc2rmelanocortin 2 receptor18Mc2rtargeted mutation 1, Dai Chida
  • glucocorticoid deficiency 1(MedGEN)
    • neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)
  • glucocorticoid deficiency 1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal adrenal gland capsule morphology(MP:0013565)

  • abnormal adrenal gland morphology(MP:0000639)

  • abnormal adrenal gland zona fasciculata morphology(MP:0008294)

  • abnormal gluconeogenesis(MP:0003383)

  • abnormal glucose homeostasis(MP:0002078)
    • more 14 phenotypes
  • adrenal gland hypoplasia(MP:0000640)

  • decreased circulating adrenaline level(MP:0005661)

  • decreased circulating aldosterone level(MP:0002667)

  • decreased circulating corticosterone level(MP:0002665)

  • decreased heart rate(MP:0005333)

  • decreased total body fat amount(MP:0010025)

  • hypoglycemia(MP:0000189)

  • increased circulating adrenocorticotropin level(MP:0001748)

  • increased circulating chloride level(MP:0003019)

  • increased circulating potassium level(MP:0005627)

  • increased spleen weight(MP:0004952)

  • increased thymus weight(MP:0004955)

  • lethargy(MP:0005202)

  • postnatal lethality, incomplete penetrance(MP:0011086)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase (PGK) promoter, E. coli Neomycin resistance gene, mouse Mc2r genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A.,104, 18205-18210 (2007). Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use.
    DepositorYoichiro Iwakura (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data