Strain Data Sheet

RBRC04905

Strain Information

Image
BRC No.RBRC04905
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameC;129P2(Cg)-Cxcr4<tm1Yiw> Tg(Lck-cre)1Jtak
Former Common nameC.129P2(Cg)-Cxcr4<tm1Yiw> Tg(Lck-cre)1Jtak
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentCxcr4 floxed mice were developed by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2010. Lck-cre Tg mice were developed by Junji Takeda, Osaka University in 1998. Double mutant mice were backcrossed BALB/c for at least 8 times.
Strain descriptionCxcr4 floxed mice with Lck-cre transgene.
Colony maintenance
References
Functional competence of T cells in the absence of glycosylphosphatidylinositol-anchored proteins caused by T cell-specific disruption of the Pig-a gene.
Takahama Y, Ohishi K, Tokoro Y, Sugawara T, Yoshimura Y, Okabe M, Kinoshita T, Takeda J
Eur. J. Immunol., 28(7):2159-2166 (1998). 9692885

CXC chemokine receptor 4 expressed in T cells plays an important role in the development of collagen-induced arthritis.
Chung S H, Seki K, Choi B I, Kimura K B, Ito A, Fujikado N, Saijo S, Iwakura Y
Arthritis Res. Ther., 12, R188 (2010). 20939892

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cxcr4C-X-C motif chemokine receptor 41Cxcr4targeted mutation 1, Yoichiro Iwakura
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)

  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • loxPphage P1 loxP1loxP
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)

  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • loxPphage P1 loxP1loxP
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)

  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)

  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • crePhage P1 Cre recombinaseUNcremouse lck promoter
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)

  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal leukocyte migration(MP:0003156)

  • decreased susceptibility to induced arthritis(MP:0003436)

  • decreased thymocyte number(MP:0000715)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Cxcr4 genomic DNA, mouse Lck promoter, phage P1 Cre recombinase gene
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arthritis Res. Ther., 12, R188 (2010). Eur. J. Immunol., 28(7):2159-2166 (1998).Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
    DepositorYoichiro Iwakura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data