Strain Data Sheet

RBRC04904

Information1

Image
BRC No.RBRC04904
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Cxcr4<tm1Yiw>
Former Common nameB6.129P2-Cxcr4<tm1Yiw>
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2010. The mice were backcrossed to C57BL/6 for at least 10 times.
Strain descriptionCxcr4 floxed mice. Systemic null knockout mice are embryonic lethal.
Colony maintenance
ReferencesArthritis Res. Ther., 12, R188 (2010). 20939892

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cxcr4chemokine (C-X-C motif) receptor 41Cxcr4<tm1Yiw>targeted mutation 1, Yoichiro Iwakura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 phage loxP sites, mouse Phosphoglycerate kinase (PGK) promoter, E. coli neomycin resistance gene, mouse Cxcr4 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arthritis Res. Ther., 12, R188 (2010). Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use.
DepositorYoichiro Iwakura (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data