Strain Data Sheet

RBRC04904

Strain Information

Image
BRC No.RBRC04904
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Cxcr4<tm1Yiw>
Former Common nameB6.129P2-Cxcr4<tm1Yiw>
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo in 2010. The mice were backcrossed to C57BL/6 for at least 10 times.
Strain descriptionCxcr4 floxed mice. Systemic null knockout mice are embryonic lethal.
Colony maintenance
References
CXC chemokine receptor 4 expressed in T cells plays an important role in the development of collagen-induced arthritis.
Chung S H, Seki K, Choi B I, Kimura K B, Ito A, Fujikado N, Saijo S, Iwakura Y
Arthritis Res. Ther., 12, R188 (2010). 20939892

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cxcr4C-X-C motif chemokine receptor 41Cxcr4targeted mutation 1, Yoichiro Iwakura
  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • loxPphage P1 loxP1loxP
  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • loxPphage P1 loxP1loxP
  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)
    • neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)
  • WHIM syndrome 1(MedGEN)

  • WHIM syndrome(DisGeNET)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal leukocyte migration(MP:0003156)

  • decreased susceptibility to induced arthritis(MP:0003436)

  • decreased thymocyte number(MP:0000715)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, mouse Phosphoglycerate kinase (PGK) promoter, E. coli neomycin resistance gene, mouse Cxcr4 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arthritis Res. Ther., 12, R188 (2010). Please obtain the written consent from Professor Yoichiro Iwakura (iwakura@rs.tus.ac.jp; Research Institute for Biological Sciences, Tokyo University of Science) for the use.
    DepositorYoichiro Iwakura (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data