RBRC04895

Information1

Image
BRC No.RBRC04895
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Timd4<tm1Osa>
Former Common nameB6-Tim4 KI/Venus
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Masanori Miyanishi and Shigekazu Nagata, Graduate School of Medicine, Kyoto University in 2007.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesNature, 450, 435-439 (2007). 17960135
Int. Immunol. 24(9):551-9 (2012). 22723547

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Timd4T-cell immunoglobulin and mucin domain containing 411Timd4<tm1Osa>targeted mutation 1, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)11Venus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNABacteriophage P1 loxP sites, Mouse Phosphoglycerate kinase-1 promoter, E.coli Neomycin resistance gene, Aequorea victoria Venus gene (GFP derivagtive), Simian virus 40 polyA, mouse Timd4 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 450, 435-439 (2007).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results within two (2) years after deposit of the BIOLOGICAL RESOURCE, the RECIPIENT must include the DEPOSITOR as co-authors.
DepositorShigekazu Nagata (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Approval form Form V (English)
Lab HP

BRC mice in Publications

No Data