Strain Data Sheet

RBRC04895

Strain Information

Image
BRC No.RBRC04895
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Timd4<tm1Osa>
Former Common nameB6-Tim4 KI/Venus
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Masanori Miyanishi and Shigekazu Nagata, Graduate School of Medicine, Kyoto University in 2007.
Strain descriptionOriginal reference: Miyanishi M, et al., Synergistic effect of Tim4 and MFG-E8 null mutations on the development of autoimmunity. Int Immunol. 2012 Sep;24(9):551-9
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Identification of Tim4 as a phosphatidylserine receptor.
Miyanishi M, Tada K, Koike M, Uchiyama Y, Kitamura T, Nagata S
Nature, 450, 435-439 (2007). 17960135

Synergistic effect of Tim4 and MFG-E8 null mutations on the development of autoimmunity.
Miyanishi M, Segawa K, Nagata S
Int. Immunol. 24(9):551-9 (2012). 22723547

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Timd4
MGI:2445125		T cell immunoglobulin and mucin domain containing 411Timd4<tm1Osa> targeted mutation 1, Shigekazu Nagata
Venus Yellow Fluorescent Protein (Aequorea victoria)11Venus
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • immune system phenotype(MP:0005387)

  • impaired macrophage phagocytosis(MP:0001798)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, Mouse Phosphoglycerate kinase-1 promoter, E.coli Neomycin resistance gene, Aequorea victoria Venus gene (GFP derivagtive), Simian virus 40 polyA, mouse Timd4 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 450, 435-439 (2007). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results within two (2) years after deposit of the BIOLOGICAL RESOURCE, the RECIPIENT must include the DEPOSITOR as co-authors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorShigekazu Nagata (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Chow A, Schad S, Green MD, Hellmann MD, Allaj V, Ceglia N, Zago G, Shah NS, Sharma SK, Mattar M, Chan J, Rizvi H, Zhong H, Liu C, Bykov Y, Zamarin D, Shi H, Budhu S, Wohlhieter C, Uddin F, Gupta A, Khodos I, Waninger JJ, Qin A, Markowitz GJ, Mittal V, Balachandran V, Durham JN, Le DT, Zou W, Shah SP, McPherson A, Panageas K, Lewis JS, Perry JSA, de Stanchina E, Sen T, Poirier JT, Wolchok JD, Rudin CM, Merghoub T.
    Tim-4+ cavity-resident macrophages impair anti-tumor CD8+ T cell immunity.
    Cancer Cell 39(7) 973-988.e9(2021) 34115989
    Lee SA, Kim D, Min C, Moon B, Lee J, Moon H, Yang S, Lee CS, Lee G, Park D.
    Phagocyte Chemoattraction Is Induced through the Mcp-1-Ccr2 Axis during Efferocytosis.
    Cells 10(11) (2021) 34831339