Strain Data Sheet
RBRC04895
Information1 | |
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| Image | |
| BRC No. | RBRC04895 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6(Cg)-Timd4<tm1Osa> |
| Former Common name | B6-Tim4 KI/Venus |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Masanori Miyanishi and Shigekazu Nagata, Graduate School of Medicine, Kyoto University in 2007. |
| Strain description | Original reference: Miyanishi M, et al., Synergistic effect of Tim4 and MFG-E8 null mutations on the development of autoimmunity. Int Immunol. 2012 Sep;24(9):551-9 |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6J(Crlj)] |
| References | Nature, 450, 435-439 (2007). 17960135 Int. Immunol. 24(9):551-9 (2012). 22723547 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Timd4T-cell immunoglobulin and mucin domain containing 411Timd4<tm1Osa>targeted mutation 1, Shigekazu Nagata Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)11VenusVenus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | Bacteriophage P1 loxP sites, Mouse Phosphoglycerate kinase-1 promoter, E.coli Neomycin resistance gene, Aequorea victoria Venus gene (GFP derivagtive), Simian virus 40 polyA, mouse Timd4 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 450, 435-439 (2007).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results within two (2) years after deposit of the BIOLOGICAL RESOURCE, the RECIPIENT must include the DEPOSITOR as co-authors. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). |
| Depositor | Shigekazu Nagata (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Chow A, Schad S, Green MD, Hellmann MD, Allaj V, Ceglia N, Zago G, Shah NS, Sharma SK, Mattar M, Chan J, Rizvi H, Zhong H, Liu C, Bykov Y, Zamarin D, Shi H, Budhu S, Wohlhieter C, Uddin F, Gupta A, Khodos I, Waninger JJ, Qin A, Markowitz GJ, Mittal V, Balachandran V, Durham JN, Le DT, Zou W, Shah SP, McPherson A, Panageas K, Lewis JS, Perry JSA, de Stanchina E, Sen T, Poirier JT, Wolchok JD, Rudin CM, Merghoub T. Tim-4+ cavity-resident macrophages impair anti-tumor CD8+ T cell immunity. Cancer Cell 39(7) 973-988.e9(2021) 34115989 Lee SA, Kim D, Min C, Moon B, Lee J, Moon H, Yang S, Lee CS, Lee G, Park D. Phagocyte Chemoattraction Is Induced through the Mcp-1-Ccr2 Axis during Efferocytosis. Cells 10(11) (2021) 34831339 |