RBRC04894

Information1

Image
BRC No.RBRC04894
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Cdc42ep4<tm1Ksh>
Former Common nameB6-Cdc42ep4 floxed
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Makoto Kinoshita, Nagoya University Graduate School of Science in 2009.
Strain description
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] Homozygous mutant mice are viable and fertile.
ReferencesNat. Commun., 6:10090 (2015). 26657011

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cdc42ep4CDC42 effector protein (Rho GTPase binding) 411Cdc42ep4 <tm1Ksh>targeted mutation 1, Makoto Kinoshita

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 phage loxP sites, Yeast FRT (flippase recombination target) sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase PolyA signal, mouse Cdc42ep4 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun. 6:10090 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorMakoto Kinoshita (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)

BRC mice in Publications

No Data