Strain Data Sheet

RBRC04892

Strain Information

Image
BRC No.RBRC04892
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Aicda<tm2.1Hon>
Former Common nameB6-AID KI/G23S
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Min Wei and Tasuku Honjo, Gradutate School of Medicine, Kyoto University in 2007.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Mice carrying a knock-in mutation of Aicda resulting in a defect in somatic hypermutation have impaired gut homeostasis and compromised mucosal defense.
Wei M, Shinkura R, Doi Y, Maruya M, Fagarasan S, Honjo T
Nat. Immunol., 12, 264-270 (2011). 21258321

Separate domains of AID are required for somatic hypermutation and class-switch recombination.
Shinkura R, Ito S, Begum N A, Nagaoka H, Muramatsu M, Kinoshita K, Sakakibara Y, Hijikata H, Honjo T
Nat. Immunol., 5, 707-712 (2004). 15195091

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aicdaactivation-induced cytidine deaminase6Aicdatargeted mutation 2, Tasuku Honjo
  • hyper-IgM syndrome type 2(MedGEN)
    • loxPphage P1 loxP6loxP
  • hyper-IgM syndrome type 2(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal gut flora balance(MP:0010377)

  • abnormal somatic hypermutation frequency(MP:0004815)

  • decreased IgG1 level(MP:0008495)

  • immune system phenotype(MP:0005387)

  • increased germinal center B cell number(MP:0008177)
    • more 2 phenotypes
  • increased susceptibility to bacterial infection induced morbidity/mortality(MP:0009788)

  • increased susceptibility to bacterial infection(MP:0002412)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Aicda (G23S) genomic DNA
    Research applicationCre/loxP system
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Immunol., 12, 264-270 (2011).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data