Strain Data Sheet



BRC No.RBRC04885
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Cacna1e<tm1Ttan>
Former Common nameB6-Cacna1e KO
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Tsutomu Tanabe, Graduate School of Medicine, Tokyo Medical and Dental University and Tetsuo Noda, The Cancer Institute, Japanese Foundation for Cancer Research in 1997. The Cacna1e gene was disrupted by insertion of a lacZ-neo cassette.
Strain descriptionCacna1e knockout mice. Homozygous and heterozygous mutant mice show reduced responses to somatic inflammatory pain. Heterozygous mutant mice also show reduced respose to visceral inflammatory pain.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 97, 6132-6137 (2000). 10801976

Health Report

Examination Date / Room / Racknull


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cacna1ecalcium channel, voltage-dependent, R type, alpha 1E subunit1Cacna1e <tm1Ttan>targeted mutation 1, Tsutomu Tanabe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, mouse Phosphoglycerate Kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Cacna1e genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci USA, 97, 6132-6137 (2000).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
DepositorTsutomu Tanabe (Tokyo Medical and Dental University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Min C, Park J, Kim G, Moon H, Lee SA, Kim D, Moon B, Yang S, Lee J, Kim K, Cho H, Park J, Lee DH, Lee G, Park D.
Tim-4 functions as a scavenger receptor for phagocytosis of exogenous particles.
Cell Death Dis 11(7) 561(2020) 32703939

Parajuli LK, Nakajima C, Kulik A, Matsui K, Schneider T, Shigemoto R, Fukazawa Y.
Quantitative regional and ultrastructural localization of the Ca(v)2.3 subunit of R-type calcium channel in mouse brain.
J Neurosci 32(39) 13555-67(2012) 23015445