Strain Data Sheet

RBRC04884

Strain Information

Image
BRC No.RBRC04884
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Cacna1b<tm1Ttan>
Former Common nameB6-Cacna1b KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Tsutomu Tanabe, Graduate School of Medicine, Tokyo Medical and Dental University in 1998. The Cacna1b was disrupted by replacement of exon 1 with an in-frame lacZ-neo cassette.
Strain descriptionCacna1b knockout mice. Homozygous mutant mice show suppressed responses to a painful stimulus that induces inflammation and markedly reduced symptoms of neuropathic pain caused by nerve injury. This strain is useful for investigating the function of pain-transmission pathway.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl] Homozygous mice show partial lethality, and display anxiety-like behavior.
References
Suppression of inflammatory and neuropathic pain symptoms in mice lacking the N-type Ca2+ channel.
Saegusa H, Kurihara T, Zong S, Kazuno A, Matsuda Y, Nonaka T, Han W, Toriyama H, Tanabe T
EMBO J., 20, 2349-2356 (2001). 11350923

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cacna1bcalcium channel, voltage-dependent, N type, alpha 1B subunit2Cacna1btargeted mutation 1, Tsutomu Tanabe
  • neurodevelopmental disorder with seizures and nonepileptic hyperkinetic movements(MedGEN)
    • lacZbeta-galactosidase (E. coli)2
  • neurodevelopmental disorder with seizures and nonepileptic hyperkinetic movements(MedGEN)
    • neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)
  • neurodevelopmental disorder with seizures and nonepileptic hyperkinetic movements(MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)2nls
  • neurodevelopmental disorder with seizures and nonepileptic hyperkinetic movements(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal behavioral response to xenobiotic(MP:0009745)

  • abnormal excitatory postsynaptic potential(MP:0002912)

  • decreased anxiety-related response(MP:0001364)

  • decreased startle reflex(MP:0001489)

  • hyporesponsive to tactile stimuli(MP:0005498)
    • more 3 phenotypes
  • increased chemical nociceptive threshold(MP:0008531)

  • nervous system phenotype(MP:0003631)

  • preweaning lethality, incomplete penetrance(MP:0011110)
    • Detailed phenotype data

    Ordering Information

    Donor DNASV40 large T antigen nuclear localization signal, E. coli lacZ, mouse Phosphoglycerate Kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Cacna1b genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 20, 2349-2356 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
    DepositorTsutomu Tanabe (Tokyo Medical and Dental University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data