Strain Data Sheet

RBRC04880

Strain Information

Image
BRC No.RBRC04880
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Gp2<tm1Soka>
Former Common nameB6-GP2 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDevelop by Shin-Ichi Fukuoka, Aoyama Gakuin University in 2003. Exon 3 and 4 of Gp2 gene was replaced with a PGK-geo selection cassette.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]. Homozygous mutant mice are viable and fertile.
References
GP2/THP gene family of self-binding, GPI-anchored proteins forms a cluster at chromosome 7F1 region in mouse genome.
Kobayashi K, Yanagihara K, Ishiguro K, Fukuoka S
Biochem. Biophys. Res. Commun., 322, 659-664 (2004). 15325280

Uptake through glycoprotein 2 of FimH(+) bacteria by M cells initiates mucosal immune response.
Hase K, Kawano K, Nochi T, Pontes G S, Fukuda S, Ebisawa M, Kadokura K, Tobe T, Fujimura Y, Kawano S, Yabashi A, Waguri S, Nakato G, Kimura S, Murakami T, Iimura M, Hamura K, Fukuoka S, Lowe A W, Itoh K, Kiyono H, Ohno H
Nature., 462, 226-230 (2009). 19907495

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gp2glycoprotein 2 zymogen granule membrane7Gp2targeted mutation 1, S Fukuoka
lacZbeta-galactosidase (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)
loxPphage P1 loxP7loxP
loxPphage P1 loxP7loxP
neoneomycin resistance gene (E. coli)7

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal immune system physiology(MP:0001790)

  • abnormal susceptibility to bacterial infection(MP:0020186)

  • decreased IgA level(MP:0001807)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli beta-galactosidase gene, E. coli neomycin resistance gene, mouse GP2 (Glycoprotein 2) genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShinichi Fukuoka (Aoyama Gakuin University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2011

    BRC mice in Publications

    No Data