Strain Data Sheet

RBRC04880

Information1

Image
BRC No.RBRC04880
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Gp2<tm1Soka>
Former Common nameB6-GP2 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDevelop by Shin-Ichi Fukuoka, Aoyama Gakuin University in 2003. Exon 3 and 4 of Gp2 gene was replaced with a PGK-geo selection cassette.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]. Homozygous mutant mice are viable and fertile.
ReferencesBiochem. Biophys. Res. Commun., 322, 659-664 (2004). 15325280
Nature., 462, 226-230 (2009). 19907495

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gp2glycoprotein 2 (zymogen granule membrane)7Gp2<tm1Soka>targeted mutation 1, S Fukuoka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;

Information2

Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli beta-galactosidase gene, E. coli neomycin resistance gene, mouse GP2 (Glycoprotein 2) genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShinichi Fukuoka (Aoyama Gakuin University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Mouse of the Month Aug 2011
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data