RBRC04878

Information1

Image
BRC No.RBRC04878
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;129-Tg(CMV-EGFP,U6-shNanog)1Ttada
Former Common nameNRi-Tg
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Shinpei Yamaguchi and Takashi Tada, Institute for Frontier Medical Sciences, Kyoto University in 2007.
Strain description
Colony maintenanceCarrier x Carrier
ReferencesDevelopment, 136, 4011-4020 (2009). 19906868

Health Report

Examination Date / Room / Rack2018/06/04Room:3-2Rack:A
2018/04/02Room:3-2Rack:A
2018/02/05Room:3-2Rack:A
2017/12/04Room:3-2Rack:A
2017/10/02Room:3-2Rack:A
2017/08/21Room:3-2Rack:A
2017/06/05Room:3-2Rack:A
2017/04/03Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
NanogNanog homeoboxUNNanogmouse U6 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFPCMV promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
TATAloxphage P1 loxP (modified loxP site)UNTATAlox

Gene symbolGene nameChr.Allele symbolAllele namePromoter
TATAloxphage P1 loxP (modified loxP site)UNTATAlox

Information2

Donor DNA5' LTR, HIV PRE, FLAP, mouse U6 promoter, Phage P1 TATAlox sites (modified loxP site), Cytomegalovirus CMV promoter, jellyfish EGFP cDNA, Nanog shRNA, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), 3' SIN LTR [update April 12, 2019]
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorTakashi Tada (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

Chen CL, Uthaya Kumar DB, Punj V, Xu J, Sher L, Tahara SM, Hess S, Machida K.
NANOG Metabolically Reprograms Tumor-Initiating Stem-like Cells through Tumorigenic Changes in Oxidative Phosphorylation and Fatty Acid Metabolism.
Cell Metab. 23(1) 206-219(2015) 26724859