Strain Data Sheet
RBRC04874
Information1 | |
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| Image | |
| BRC No. | RBRC04874 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Gt(ROSA)26Sor<tm1(CAG-EGFP/tDsRed)Utr>/Rbrc |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | B6N-22<UTR> [C57BL/6NCrlCrlj] |
| Background strain | C57BL/6NCrlCrlj |
| Appearance | |
| Strain development | Developed by Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center in 2011. B6N-22<Ttr> ES cells were used. C57BL/6N background. |
| Strain description | R26GRR knock-in mice. A construct consisting of the EGFP-tdsRed (tandem version of dimeric DsRed) under the control of the CAG promoter, was inserted via homologous recombination into the intron between exons1 and 2 of the ROSA26 locus. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrl] |
| References | Exp. Anim., 62, 295-304 (2013). 24172193 Exp. Anim., 71(3), 385-390 (2022). 35444103 |
Health Report | |
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| Examination Date / Room / Rack | 2023/01/10Room:3-BRack:F 2022/10/11Room:3-BRack:F 2022/07/11Room:3-BRack:F 2022/04/11Room:3-BRack:F 2022/01/24Room:3-BRack:F 2021/10/26Room:3-BRack:F 2021/07/26Room:3-BRack:F 2021/04/26Room:3-BRack:F |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA26)Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA26)Sor<tm1(CAG-EGFP/DsRed)Utr>targeted mutation 1 (CAG-EGFP/DsRed), University of Tsukuba Laboratory Animal Resource Center Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)6FLAG Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tDsRedRed Fluorescent Protein (Discosoma sp.)6tDsRed Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Information2 | |
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| Donor DNA | Rosa gene (ubiquitous) (mouse), CMV IE enhancer (cytomegalovirus), beta-actin promoter (chicken), beta-globin genomic DNA (rabbit), EGFP (jellyfish), DsRed (red flourescent protein from Discosoma sp.) cDNA, PGK promoter (mouse), neomycin resistance gene (E. coli), polyA additional signal (mouse), FRT sites (yeast), loxP sites (P1 phage), phage T7 gb2 promoter(synthetic), polyA (SV40), FLAG tag (synthetic) |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp Anim 62, 295-304 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | RIKEN BRC (RIKEN BRC) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
| Additional Info. | Mouse of the Month Mar 2014, Mouse of the Month Oct 2018 Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Hashimoto M, Sasaki H. Epiblast Formation by TEAD-YAP-Dependent Expression of Pluripotency Factors and Competitive Elimination of Unspecified Cells. Dev Cell 50(2) 139-154.e5(2019) 31204175 Shibata M, Banno R, Sugiyama M, Tominaga T, Onoue T, Tsunekawa T, Azuma Y, Hagiwara D, Lu W, Ito Y, Goto M, Suga H, Sugimura Y, Oiso Y, Arima H. AgRP Neuron-Specific Deletion of Glucocorticoid Receptor Leads to Increased Energy Expenditure and Decreased Body Weight in Female Mice on a High-Fat Diet. Endocrinology 157(4) 1457-66(2016) 26889940 Matsumoto L, Hirota Y, Saito-Fujita T, Takeda N, Tanaka T, Hiraoka T, Akaeda S, Fujita H, Shimizu-Hirota R, Igaue S, Matsuo M, Haraguchi H, Saito-Kanatani M, Fujii T, Osuga Y. HIF2α in the uterine stroma permits embryo invasion and luminal epithelium detachment. J Clin Invest 128(7) 3186-3197(2018) 29911998 Hiraoka T, Hirota Y, Saito-Fujita T, Matsuo M, Egashira M, Matsumoto L, Haraguchi H, Dey SK, Furukawa KS, Fujii T, Osuga Y. STAT3 accelerates uterine epithelial regeneration in a mouse model of decellularized uterine matrix transplantation. JCI Insight 1(8) (2016) 27358915 Mizoguchi A, Banno R, Sun R, Yaginuma H, Taki K, Kobayashi T, Sugiyama M, Tsunekawa T, Onoue T, Takagi H, Hagiwara D, Ito Y, Iwama S, Suga H, Nagai T, Yamada K, Arima H. Glucocorticoid receptor signaling in ventral tegmental area neurons increases the rewarding value of a high-fat diet in mice. Sci Rep 11(1) 12873(2021) 34145364 Tsunekawa T, Banno R, Yaginuma H, Taki K, Mizoguchi A, Sugiyama M, Onoue T, Takagi H, Hagiwara D, Ito Y, Iwama S, Goto M, Suga H, Bettler B, Arima H. GABAB Receptor Signaling in the Mesolimbic System Suppresses Binge-like Consumption of a High-Fat Diet. iScience 20 337-347(2019) 31610370 |