RBRC04874

Information1

Image
BRC No.RBRC04874
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Gt(ROSA)26Sor<tm1(CAG-EGFP/tDsRed)Utr>/Rbrc
Former Common name
H-2 Haplotype
ES Cell lineB6N-22<UTR> [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center in 2011. B6N-22<Ttr> ES cells were used. C57BL/6N background.
Strain descriptionR26GRR knock-in mice. A construct consisting of the EGFP-tdsRed (tandem version of dimeric DsRed) under the control of the CAG promoter, was inserted via homologous recombination into the intron between exons1 and 2 of the ROSA26 locus.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesExp Anim 62, 295-304 (2013). 24172193

Health Report

Examination Date / Room / Rack2020/06/01Room:3-BRack:F
2020/02/17Room:3-BRack:F
2019/12/16Room:3-BRack:F
2019/10/21Room:3-BRack:F
2019/08/26Room:3-BRack:F
2019/06/17Room:3-BRack:F
2019/04/15Room:3-BRack:F
2019/02/18Room:3-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA26)Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA26)Sor<tm1(CAG-EGFP/DsRed)Utr>targeted mutation 1 (CAG-EGFP/DsRed), University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)6EGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tdsRedRed Fluorescent Protein (Discosoma sp.)6tdsRed

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA6

Information2

Donor DNARosa gene (ubiquitous) (mouse), CMV IE enhancer (cytomegalovirus), beta-actin promoter (chicken), beta-globin genomic DNA (rabbit), EGFP (jellyfish), DsRed (red flourescent protein from Discosoma sp.) cDNA, PGK promoter (mouse), neomycin resistance gene (E. coli), polyA additional signal (mouse), FRT sites (yeast), loxP sites (P1 phage), phage T7 gb2 promoter(synthetic), polyA (SV40), FLAG tag (synthetic)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp Anim 62, 295-304 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Mar 2014, Mouse of the Month Oct 2018
Category I MTA: FP license of TBUSA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

Shibata M, Banno R, Sugiyama M, Tominaga T, Onoue T, Tsunekawa T, Azuma Y, Hagiwara D, Lu W, Ito Y, Goto M, Suga H, Sugimura Y, Oiso Y, Arima H.
AgRP Neuron-Specific Deletion of Glucocorticoid Receptor Leads to Increased Energy Expenditure and Decreased Body Weight in Female Mice on a High Fat Diet.
Endocrinology (2016) 26889940

Matsumoto L, Hirota Y, Saito-Fujita T, Takeda N, Tanaka T, Hiraoka T, Akaeda S, Fujita H, Shimizu-Hirota R, Igaue S, Matsuo M, Haraguchi H, Saito-Kanatani M, Fujii T, Osuga Y.
HIF2α in the uterine stroma permits embryo invasion and luminal epithelium detachment.
J. Clin. Invest. (2018) 29911998

Takehiro Hiraoka, Yasushi Hirota, Tomoko Saito-Fujita, Mitsunori Matsuo, Mahiro Egashira, Leona Matsumoto, Hirofumi Haraguchi, Sudhansu K. Dey, Katsuko S. Furukawa, Tomoyuki Fujii, and Yutaka Osuga.
STAT3 accelerates uterine epithelial regeneration in a mouse model of decellularized uterine matrix transplantation.
JCI Insight (2016) 27358915