RBRC04867

Information1

Image
BRC No.RBRC04867
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(IGHEmu/VH-preMir125b-1)116Wakh
Former Common nameB6-Emu-miRNA125 Tg#116
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takashi Sonoki, Department of Hematology and Oncology, Wakayama Medical University in 2009. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. The transgene was integrated into the chromosome 15 in the line 116, chromosome 6 in the line 005-I (005-1), and chromosome 5 in the line 005-II (005-2), respectively. Line 116 (RBRC04867), Line 005-I (RBRC04868), Line 005-II (RBRC04869).
Strain descriptionThese transgenic mice express a mouse microRNA129b-1 (precursor) under the control of the Human IGHEmu enhancer/mouse VH promoter. Tg mice exhibit IgM negative B-cell neoplasms, and die within 15-30 weeks of age in the line 116, 30-60 weeks of age in the line 005-I (005-1), and 30-45 weeks of age in the line 005-II (005-2), respectively.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesLeukemia. 2011 Dec;25(12):1849-56. doi: 10.1038/leu.2011.166. Epub 2011 Jul 8. 21738213

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mir125b-1microRNA 125b-1UNMir125b-1human immunoglobulin heavy chain enhancer, mouse immunoglobulin heavy chain VH promoter

Information2

Donor DNAヒト 免疫グロブリン重鎖遺伝子内因性エンハンサー (Emu), マウス 免疫グロブリン重鎖遺伝子VHプロモーター, ラット beta―グロビン遺伝子エクソンII, イントロン, エクソンIII, マウス Pre-miR125-b-1断片
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Leukemia. 2011 Dec;25(12):1849-56. doi: In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
DepositorTakashi Sonoki (Wakayama Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data