Strain Data Sheet

RBRC04855

Strain Information

Image
BRC No.RBRC04855
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Vamp7<tm2Aha>
Former Common nameB6-Vamp7 KO#2
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Akihiro Harada, Institute for Molecular and Cellular Regulation, Gunma University in 2005. Two lines were generated (RBRC01926 and RBRC04855).
Strain descriptionVamp7 floxed mice (Neo type). Exons 3 and 4 of the Vamp7 gene were flanked by loxP sites, an FRT-SA-Ex 5,6,7,8-IRES-Neo-polyA-FRT cassette was inserted between exons 4 and 5. Vamp7 is a member of VAMP (vesicle-associated membrane protein) subfamily of SNARES proteins which primarily mediate fusion of cellular transport vesicles with a target membrane, and ubiquitously expressed and in localized to the late endsome, the lysosome, and the trans-Golgi network (TGN).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
References
Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth.
Martinez-Arca S, Alberts P, Zahraoui A, Louvard D, Galli T
J. Cell Biol., 2000 May 15;149(4):889-900. 10811829

The role of VAMP7/TI-VAMP in cell polarity and lysosomal exocytosis in vivo.
Sato M, Yoshimura S, Hirai R, Goto A, Kunii M, Atik N, Sato T, Sato K, Harada R, Shimada J, Hatabu T, Yorifuji H, Harada A
Traffic., 12(10):1383-1393 (2011). 21740490

A novel tetanus neurotoxin-insensitive vesicle-associated membrane protein in SNARE complexes of the apical plasma membrane of epithelial cells.
Galli T, Zahraoui A, Vaidyanathan V V, Raposo G, Tian J M, Karin M, Niemann H, Louvard D
Mol. Biol. Cell, 9, 1437-1448 (1998). 9614185

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) siteXFrt
IRESinternal ribosomal entry site (EMCV)X
Vamp7vesicle-associated membrane protein 7XVamp7targeted mutation 2, Akihiro Harada
loxPphage P1 loxPXloxP
loxPphage P1 loxPXloxP
neoneomycin resistance gene (E. coli)X

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal axon extension(MP:0003651)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli neo, phage P1 loxP site, yeast FRT (flipase recombination target) site, mouse VAMP7 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Before the first publication on the BIOLOGICAL RESOURCE by DEPOSITOR/DEVELOPER, the RECIPIENT agrees to use this BIOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER, Within a period of Two years after the first paper concerning the analysis of BIOLOGICAL RESOURCE is published, the RECIPIENT agree use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER.
    DepositorAkihiro Harada (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data