RBRC04852

Information1

Image
BRC No.RBRC04852
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6C3(129)-Tg(NDE-hsp-Smad3DN,lacZ)1Hmd
Former Common nameNDE-dnSmad3-1Z(1)
H-2 Haplotype
ES Cell line
Background strainJcl:B6C3F1
Appearance
Strain developmentDeveloped by Aiko Kawasumi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2009 The transgene was injected into the pronuclei of (C57BL/6 x C3H) F1 fertelized eggs. C57BL/6, C3H and 129 mixed background.
Strain description
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6C3F1]
ReferencesDev. Biol., 353, 321-330 (2011). 21419113

Health Report

Examination Date / Room / Rack2012/04/16Room:3-BRack:F
2012/02/20Room:3-BRack:F
2011/12/12Room:3-BRack:F
2011/10/17Room:3-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Smad3SMAD family member 3UNSmad3mouse hsp promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Information2

Donor DNAマウス由来NodalのNDEエンハンサー dominant negative (3’ AAVA) 型に改変したSmad3 cDNA, hspプロモーター, E. coli由来LacZ遺伝子, ウィルス由来IRES配列
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.

BRC mice in Publications

No Data