RBRC04848

Information1

Image
BRC No.RBRC04848
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.B6129-Gt(ROSA)26Sor<tm1(CAG-ECFP/Venus)Kgwa>
Former Common nameB6-ROSA/SCAT3.1(floxed) KI
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Michio Tomura and Osami Kanagawa, Research Center for Allergy and Immunologuy, RIKEN in 2008.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesInt. Immunol., 21, 1145-1150 (2009). 19710208

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-ECFP/Venus)Kgwa>targeted mutation 1, Osami Kanagawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ECFPEnhanced Cyan Fluorescent Protein (Aequorea victoria), F64L/S65T/K212N/H231L6ECFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)6Venus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
STOP codon (synthetic)6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Caspase-3 cleavable peptide6

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, STOP codon (synthetic), Aequorea victria GFP derivatives (ECFP, Venus) cDNA, Caspase-3 cleavable peptide, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, mouse Gt(ROSA)26Sor region genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the SCAT3.1 using Approval form (form SCAT) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a MTA regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki, Osaka University (FAX: +81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp). In publishing the research results to be obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Int. Immunol., 21(10), 1145-1150 (2009).) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must send the DEPOSITOR a reprint of RECIPIENT's publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering: 1. Approval form (form D) from the depositor (ENG) , (JPN) , 2. Approval form (form SCAT) from the developer (ENG), 3. MTA for pCAGGS with the developer (ENG), 4. MTA for distribution with RIKEN BRC and 5. Order form (ENG) , (JPN) .
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data