Strain Data Sheet

RBRC04842

Strain Information

Image
BRC No.RBRC04842
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(Lck-Bhlhe40)1Homy
Former Common nameDec1 Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The Lck promoter drives expression of Decq cDNA carrying a FLAG tag. The transgene was injected into the pronuclei of C57BL/6N fertlized eggs.
Strain descriptionThe mice express a Dec1 (Bhlhe40) cDNA carrying a FLAG tag under the control of the Lck promoter.
Colony maintenance
References
The role of the basic helix-loop-helix transcription factor Dec1 in the regulatory T cells.
Miyazaki K, Miyazaki M, Guo Y, Yamasaki N, Kanno M, Honda Z, Oda H, Kawamoto H, Honda H
J. Immunol., 185, 7330-7339 (2010). 21057086

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bhlhe40basic helix-loop-helix family member e40UNBhlhe40mouse Lck promoter
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)
    • FLAGFLAG tag (synthetic)UNFLAG
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • increased CD4-positive, CD25-positive, alpha-beta regulatory T cell number(MP:0010168)

  • scaly skin(MP:0001192)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Lck promoter, mouse Dec1 (Bhlhe40) cDNA, FLAG tag (synthetic DNA)
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data