Strain Data Sheet

RBRC04842

Strain Information

Image
BRC No.RBRC04842
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(Lck-Bhlhe40)1Homy
Former Common nameDec1 Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The Lck promoter drives expression of Decq cDNA carrying a FLAG tag. The transgene was injected into the pronuclei of C57BL/6N fertlized eggs.
Strain descriptionThe mice express a Dec1 (Bhlhe40) cDNA carrying a FLAG tag under the control of the Lck promoter.
Colony maintenance
ReferencesJ. Immunol., 185, 7330-7339 (2010). 21057086

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bhlhe40basic helix-loop-helix family member e40UNBhlhe40mouse Lck promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Ordering Information

Donor DNAmouse Lck promoter, mouse Dec1 (Bhlhe40) cDNA, FLAG tag (synthetic DNA)
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data