Strain Data Sheet

RBRC04841

Strain Information

Image
BRC No.RBRC04841
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6N.129P2-Bhlhe40<tm1Homy>
Former Common nameDec1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The promoter and coding region including the ATG in exon1 was replaced with a neomycin resistance gene. C57BL/6N background
Strain descriptionDec1 (Bhlhe40) knockout mice. The promoter and coding region including the ATG in exon 1 was replaced with a neomycin resistance gene. Homozygous mutant mice exhibit a decrease in T cell number in aged mice and a development of lymphoproliferative autoimmune disease.
Colony maintenance
References
The role of the basic helix-loop-helix transcription factor Dec1 in the regulatory T cells.
Miyazaki K, Miyazaki M, Guo Y, Yamasaki N, Kanno M, Honda Z, Oda H, Kawamoto H, Honda H
J. Immunol., 185, 7330-7339 (2010). 21057086

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bhlhe40basic helix-loop-helix family, member e406Bhlhe40targeted mutation 1, Hiroaki Honda
neoneomycin resistance gene (Streptomyces fradiase)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal CD4-positive, alpha beta T cell morphology(MP:0002432)

  • abnormal CD8-positive, alpha beta T cell morphology(MP:0005010)

  • abnormal acute inflammation(MP:0002498)

  • absent lymph node germinal center(MP:0008523)

  • decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number(MP:0010169)
    • more 9 phenotypes
  • decreased double-positive T cell number(MP:0005092)

  • decreased regulatory T cell number(MP:0004974)

  • decreased spleen red pulp amount(MP:0008477)

  • decreased spleen white pulp amount(MP:0008479)

  • enlarged lymph nodes(MP:0000702)

  • enlarged spleen(MP:0000691)

  • increased T cell derived lymphoma incidence(MP:0002024)

  • increased double-negative T cell number(MP:0005090)

  • large lymphoid organs(MP:0002220)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), Streptomyces fradiase neomycin phosphotransferase genomic DNA, mouse Bhlhe40 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Hu J, Mao Z, He S, Zhan Y, Ning R, Liu W, Yan B, Yang J.
    Icariin protects against glucocorticoid induced osteoporosis, increases the expression of the bone enhancer DEC1 and modulates the PI3K/Akt/GSK3β/β-catenin integrated signaling pathway.
    Biochem Pharmacol 136 109-121(2017) 28408345
    Wu Y, Wang H, Huo Y, Yan B, Honda H, Liu W, Yang J.
    DIFFERENTIATED EMBRYONIC CHONDROCYTE EXPRESSED GENE-1 IS A CENTRAL SIGNALING COMPONENT IN THE DEVELOPMENT OF COLLAGEN-INDUCED RHEUMATOID ARTHRITIS.
    J Biol Chem 102982(2023) 36739947
    Lin L, Qiang Z, Chen K, Huo Y, Liu W, Yang J.
    DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation.
    J Biomed Res 1-16(2024) 38807374
    Hirata H, Kamohara A, Murayama M, Nishioka K, Honda H, Urano Y, Soejima H, Oki S, Kukita T, Kawano S, Mawatari M, Kukita A.
    A novel role of helix-loop-helix transcriptional factor Bhlhe40 in osteoclast activation.
    J Cell Physiol 237(10) 3912-3926(2022) 35908202
    Hu X, Zou M, Zheng W, Zhu M, Hou Q, Gao H, Zhang X, Liu Y, Cheng Z.
    Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis.
    Respir Res 25(1) 100(2024) 38402153