Strain Data Sheet


Strain Information

BRC No.RBRC04841
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6N.129P2-Bhlhe40<tm1Homy>
Former Common nameDec1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6NCrlCrlj
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The promoter and coding region including the ATG in exon1 was replaced with a neomycin resistance gene. C57BL/6N background
Strain descriptionDec1 (Bhlhe40) knockout mice. The promoter and coding region including the ATG in exon 1 was replaced with a neomycin resistance gene. Homozygous mutant mice exhibit a decrease in T cell number in aged mice and a development of lymphoproliferative autoimmune disease.
Colony maintenance
ReferencesJ. Immunol., 185, 7330-7339 (2010). 21057086

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bhlhe40basic helix-loop-helix family member e406Bhlhe40<tm1Homy>targeted mutation 1, Hiroaki Honda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (Streptomyces fradiase)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), Streptomyces fradiase neomycin phosphotransferase genomic DNA, mouse Bhlhe40 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Hu J, Mao Z, He S, Zhan Y, Ning R, Liu W, Yan B, Yang J.
Icariin protects against glucocorticoid induced osteoporosis, increases the expression of the bone enhancer DEC1 and modulates the PI3K/Akt/GSK3β/β-catenin integrated signaling pathway.
Biochem Pharmacol 136 109-121(2017) 28408345

Wu Y, Wang H, Huo Y, Yan B, Honda H, Liu W, Yang J.
J Biol Chem 102982(2023) 36739947

Hirata H, Kamohara A, Murayama M, Nishioka K, Honda H, Urano Y, Soejima H, Oki S, Kukita T, Kawano S, Mawatari M, Kukita A.
A novel role of helix-loop-helix transcriptional factor Bhlhe40 in osteoclast activation.
J Cell Physiol 237(10) 3912-3926(2022) 35908202

Hu X, Zou M, Zheng W, Zhu M, Hou Q, Gao H, Zhang X, Liu Y, Cheng Z.
Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis.
Respir Res 25(1) 100(2024) 38402153