Strain Data Sheet

RBRC04832

Strain Information

Image
BRC No.RBRC04832
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2(CD1)-Id4<tm1Fsky>
Former Common nameB6-Id4 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Fred Sblitzky, University of Nottingham in 2005. A lacZ-neo cassette replaced 220 bp of the ID4 cording region including the HLH domain. The mutant mice were backcrossed to C57BL/6 from 129 and CD1 (ICR) mixed background.
Strain descriptionId4 knockout mice. Id4 is a member of inhibitor of differentiation / inhibitor of DNA binding protein family. Homozygous mutant mice exhibit a abnormal brain structures.
Colony maintenanceHeterozygote X Wild-type [C57BL/6JJcl] Homozygous mutant mice are small and most of them die within 8 weeks of age.
References
Id4 is required for the correct timing of neural differentiation.
Bedford L, Walker R, Kondo T, van Cruchten I, King E R, Sablitzky F
Dev. Biol., 280, 386-395(2005). 15882580

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Id4inhibitor of DNA binding 413Id4targeted mutation 1, Fred Sablitzky
lacZbeta-galactosidase (E. coli)13
neoneomycin resistance gene (E. coli)13herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal astrocyte morphology(MP:0002182)

  • abnormal basal ganglion morphology(MP:0006007)

  • abnormal bone mineralization(MP:0002896)

  • abnormal bone ossification(MP:0008271)

  • abnormal bone structure(MP:0003795)
    • more 23 phenotypes
  • abnormal brain development(MP:0000913)

  • abnormal epiphyseal plate morphology(MP:0006395)

  • abnormal fat cell morphology(MP:0009115)

  • abnormal forebrain development(MP:0003232)

  • abnormal hippocampus development(MP:0000808)

  • abnormal lateral ganglionic eminence morphology(MP:0004277)

  • abnormal lateral ventricle morphology(MP:0000823)

  • abnormal medial ganglionic eminence morphology(MP:0004276)

  • abnormal neocortex morphology(MP:0008547)

  • abnormal neuron differentiation(MP:0009937)

  • abnormal osteoblast morphology(MP:0004986)

  • abnormal stratification in cerebral cortex(MP:0000790)

  • decreased body length(MP:0001258)

  • decreased body weight(MP:0001262)

  • decreased bone mineralization(MP:0020137)

  • decreased bone ossification(MP:0020040)

  • decreased brain size(MP:0000774)

  • postnatal growth retardation(MP:0001732)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • premature death(MP:0002083)

  • premature neuronal precursor differentiation(MP:0003424)

  • skeleton phenotype(MP:0005390)

  • weight loss(MP:0001263)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli LacZ, polyA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, polyA, mouse Id4 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 280, 386-395 (2005).The RECIPIENT must contact Prof. Fred. Sablitzky, The University of Nottingham (fred.sablitzky@nottingham.ac.uk) to obtain permission for us the BIOLOGICAL RESOURCE.
    DepositorYasushi Okazaki (Saitama Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data