RBRC04832

Information1

Image
BRC No.RBRC04832
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2(CD1)-Id4<tm1Fsky>
Former Common nameB6-Id4 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Fred Sblitzky, University of Nottingham in 2005. A lacZ-neo cassette replaced 220 bp of the ID4 cording region including the HLH domain. The mutant mice were backcrossed to C57BL/6 from 129 and CD1 (ICR) mixed background.
Strain descriptionId4 knockout mice. Id4 is a member of inhibitor of differentiation / inhibitor of DNA binding protein family. Homozygous mutant mice exhibit a abnormal brain structures.
Colony maintenanceHeterozygote X Wild-type [C57BL/6JJcl] Homozygous mutant mice are small and most of them die within 8 weeks of age.
ReferencesDev. Biol., 280, 386-395(2005). 15882580

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Id4inhibitor of DNA binding 413Id4<tm1Fsky>targeted mutation 1, Fred Sablitzky

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAE. coli LacZ, polyA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, polyA, mouse Id4 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 280, 386-395 (2005).The RECIPIENT must contact Prof. Fred. Sablitzky, The University of Nottingham (fred.sablitzky@nottingham.ac.uk) to obtain permission for us the BIOLOGICAL RESOURCE.
DepositorYasushi Okazaki (Saitama Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data