RBRC04823

Information1

Image
BRC No.RBRC04823
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcdha<tm>/TYagRbrc
Former Common nameB6;B6CB-Pcdha<tm>/TYagRbrc, Pcdha cKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Astushi Okayama, Takahiro Hirabayashi and and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2010.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesFront. Mol. Neurosci., 9:155 (2016). 28066179

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pcdhaprotocadherin alpha cluster18Pcdha<tm>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)18Venus

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
myc-tagmyc-epitope tag (human)18myc-tag

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18

Information2

Donor DNAMouse Pcdha1 genomic DNA, P1 phage loxP, S. cerevisiae FRT, E. coli Transposon Tn5 Neo, jellyfish Venus cDNA, human Myc
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data