Strain Data Sheet

RBRC04823

Strain Information

Image
BRC No.RBRC04823
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcdha<tm>/TYagRbrc
Former Common nameB6;B6CB-Pcdha<tm>/TYagRbrc, Pcdha cKO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Astushi Okayama, Takahiro Hirabayashi and and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2010.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
References
Distinct and Cooperative Functions for the Protocadherin-alpha, -beta and -gamma Clusters in Neuronal Survival and Axon Targeting.
Hasegawa S, Kumagai M, Hagihara M, Nishimaru H, Hirano K, Kaneko R, Okayama A, Hirayama T, Sanbo M, Hirabayashi M, Watanabe M, Hirabayashi T, Yagi T
Front. Mol. Neurosci., 9:155 (2016). 28066179

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site18Frt
Pcdhaprotocadherin alpha cluster18Pcdhatargeted mutation
VenusYellow Fluorescent Protein (Aequorea victoria)18Venus
loxPphage P1 loxP18loxP
myc-tagmyc-epitope tag (human)18myc-tag
neoneomycin resistance gene (E. coli)18

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Pcdha1 genomic DNA, P1 phage loxP, S. cerevisiae FRT, E. coli Transposon Tn5 Neo, jellyfish Venus cDNA, human Myc
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data