Strain Data Sheet

RBRC04820

Strain Information

Image
BRC No.RBRC04820
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Del(18Pcdha-Pcdhg) Tg(Taf7)/TyagRbrc
Former Common nameDelta Pcdhabg
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2010.
Strain descriptionKO homozygous mutant mice are embryonic lethal. KO homozygous mutant mice with Tg allele are viable.
Colony maintenanceKO : Homozygote X Heterozygote ; Tg : Carrier X Carrier [or Crossing to C57BL/6J(Crlj)]
References
Distinct and Cooperative Functions for the Protocadherin-alpha, -beta and -gamma Clusters in Neuronal Survival and Axon Targeting.
Hasegawa S, Kumagai M, Hagihara M, Nishimaru H, Hirano K, Kaneko R, Okayama A, Hirayama T, Sanbo M, Hirabayashi M, Watanabe M, Hirabayashi T, Yagi T
Front. Mol. Neurosci., 9:155 (2016). 28066179

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pcdhaprotocadherin alpha cluster18Pcdha
Pcdhgprotocadherin gamma cluster18Pcdhg
loxPphage P1 loxP18loxP
Taf7TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factorUNTaf7transgene insertion

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal brainstem morphology(MP:0005277)

  • abnormal innervation(MP:0002184)

  • abnormal medulla oblongata morphology(MP:0000846)

  • abnormal olfactory bulb interneuron morphology(MP:0009941)

  • abnormal olfactory bulb morphology(MP:0000819)
    • more 9 phenotypes
  • abnormal postsynaptic density morphology(MP:0020551)

  • abnormal spinal cord ventral commissure morphology(MP:0009695)

  • astrocytosis(MP:0003354)

  • decreased midbrain size(MP:0012089)

  • decreased spinal cord size(MP:0000956)

  • hunched posture(MP:0001505)

  • increased neuron apoptosis(MP:0003203)

  • increased retina apoptosis(MP:0030005)

  • umbilical hernia(MP:0010146)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP site, mouse Pcdh-abg genomic DNA, mouse Taf7 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data