Strain Data Sheet

RBRC04818

Strain Information

Image
BRC No.RBRC04818
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(Nes-PhiC31*/ERT2)2Imayo
Former Common nameNes-PhiC31/ERT2 Tg#2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi, Institute for Virus Research Kyoto University in 2010. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.
Strain descriptionThis strain expresses a tamoxifen-inducible PhiC31 recombinase in neural stem / progenitor cells under the control of the Nestin promoter.
Colony maintenanceCarrier X Noncarrier [C57BL/6J(Crlj)] Tg mice are viable and fertile.
References
In vivo evaluation of PhiC31 recombinase activity in transgenic mice.
Imayoshi I, Hirano K, Kitano S, Miyachi H, Kageyama R
Neurosci. Res., 73(2), 106-114 (2012). 22608021

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2
PhiC31PhiC31 recombinaseUNPhiC31rat Nestin promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNArat Nestin promoter / Second intron, Synthetic PhiC31o, human mutant-type mouse estrogen receptor, SV40 PolyA signal
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 73(2), 106-114 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
    DepositorItaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Itaru Imayoshi HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data