RBRC04807

Information1

Image
BRC No.RBRC04807
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Spata9<tm1Osb>
Former Common nameB6-Spata9 KO
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Hide Hatayama and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2010.
Strain descriptionSpata9 gene knockout mice. spata9, spermatogenesis associated 9 is a gene specifically expressing in testis.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NJcl]
ReferencesProc. Natl. Acad. Sci. USA, 113, 7704-7710 (2016). 27357688

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Spata9spermatogenesis associated 913Spata9<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site13Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site13Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse PGK pA signal, phage P1 loxP site, yeast FRT (flipase recombination target) sites, mouse Spata9 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A., 113, 7704-7710 (2016).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data