Strain Data Sheet

RBRC04801

Strain Information

Image
BRC No.RBRC04801
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Kcp<tm1Ysas>/Rbrc
Former Common nameKcp(tau-lacZ) (Acc.No.CDB0401K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Yoshiki Sasai, Center for Developmental Biology, RIKEN.
Strain descriptionThis transgene contains tau-lacZ with a floxed neo in the first exon of Kcp to replace with lacZ at the first ATG. Kcp is a Kielin-related molecule that contains multiple Chd-type cysteine-rich repeats, a vWD domain and a TIL domain, and physically binds to Bmp proteins. Homozygous mutant mice are viable and fertile, and exhibit no gross structural defects.
Colony maintenanceHeterozygote X Wild-type [C56BL/6] Homozygous mutant mice are viable and fertile.
References
Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis.
Ikeya M, Kawada M, Kiyonari H, Sasai N, Nakao K, Furuta Y, Sasai Y
Development, 133, 4463-4473 (2006). 17035289

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Kcpkielin/chordin-like protein6Kcptargeted mutation 1, Yoshiki Sasai
lacZbeta-galactosidase (E. coli)6
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)
poly ASV40 polyA6poly A
taubovine tau protein cDNA6tau

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAbovine tau cDNA, E. coli lacZ, SV40 polyA, phage P1 loxP sites, mouse PGK promoter, E. coli neo, mouse Kcp genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Makoto Ikeya (mikeya@cira.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0401K, and Web Site (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Development, 133, 4463-4473 (2006).) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data