Strain Data Sheet

RBRC04791

Strain Information

Image
BRC No.RBRC04791
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Dapk1<tm1Yuk>
Former Common nameB6;129P2-Dapk1<tm1Yuk>, DAPK KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Kazunori Yukawa, Wakayama Medical University and Dr. Sizuo Akira, Research Institute for Microbial Disease, Osaka University in 2004. C57BL/6J background.
Strain descriptionDapk1 gene knockout mice. Exon encoding amino acids 22-95 of Dapk1 gene was replaced with a neomycin resistant cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]Homozygous mutant mice are viable and fertile, but females of homozygote show slightly lower reproductive performance.
References
Deletion of the kinase domain in death-associated protein kinase attenuates tubular cell apoptosis in renal ischemia-reperfusion injury.
Kishino M, Yukawa K, Hoshino K, Kimura A, Shirasawa N, Otani H, Tanaka T, Owada-Makabe K, Tsubota Y, Maeda M, Ichinose M, Takeda K, Akira S, Mune M
J. Am. Soc. Nephrol., 15, 1826-1834 (2004). 15213270

Irinotecan-induced ovarian follicular apoptosis is attenuated by deleting the kinase domain of death-associated protein kinase.
Li L, Tanaka T, Yukawa K, Akira S, Umesaki N
Int. J. Oncol., 34, 905-914 (2009). 19287947

Deletion of the kinase domain in death-associated protein kinase attenuates renal tubular cell apoptosis in chronic obstructive uropathy.
Yukawa K, Hoshino K, Kishino M, Mune M, Shirasawa N, Kimura A, Tsubota Y, Owada-Makabe K, Tanaka T, Ichinose M, Maeda M, Takeda K, Akira S
Int. J. Mol. Med., 13, 515-520 (2004). 15010850

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dapk1death associated protein kinase 113Dapk1targeted mutation 1, Kazunori Yukawa
neoneomycin resistance gene (E. coli)13herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased renal tubule apoptosis(MP:0011372)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHerpes simplex virus Thymidine kinase promoter, E. coli Neomycin resistance gene, Herpes simplex virus Thymidine kinase polyA, mouse Dapk1 genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. J. Mol. Med., 13, 515-520 (2004).
    DepositorMasayasu Miyajima (Wakayama Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data