RBRC04791

Information1

Image
BRC No.RBRC04791
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Dapk1<tm1Yuk>
Former Common nameB6;129P2-Dapk1<tm1Yuk>, DAPK KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Kazunori Yukawa, Wakayama Medical University and Dr. Sizuo Akira, Research Institute for Microbial Disease, Osaka University in 2004. C57BL/6J background.
Strain descriptionDapk1 gene knockout mice. Exon encoding amino acids 22-95 of Dapk1 gene was replaced with a neomycin resistant cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]Homozygous mutant mice are viable and fertile, but females of homozygote show slightly lower reproductive performance.
ReferencesJ. Am. Soc. Nephrol., 15, 1826-1834 (2004). 15213270
Int. J. Oncol., 34, 905-914 (2009). 19287947
Int. J. Mol Med., 13, 515-520 (2004). 15010850

Health Report

Examination Date / Room / Rack2015/02/18Room:4-ARack:C
2015/02/16Room:4-ARack:C
2015/01/19Room:4-ARack:C
2014/11/17Room:4-ARack:C
2014/11/12Room:4-ARack:C
2014/11/10Room:4-ARack:C
2014/10/15Room:4-ARack:C
2014/09/16Room:4-ARack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dapk1death associated protein kinase 113Dapk1<tm1Yuk>targeted mutation 1, Kazunori Yukawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAHerpes simplex virus Thymidine kinase promoter, E. coli Neomycin resistance gene, Herpes simplex virus Thymidine kinase polyA, mouse Dapk1 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. J. Mol. Med., 13, 515-520 (2004).
DepositorMasayasu Miyajima (Wakayama Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data