Strain Data Sheet

RBRC04790

Strain Information

Image
BRC No.RBRC04790
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Capn9<tm1Hiso>
Former Common name
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Drs. Shoji Hata and Hiroyuki Sorimachi, The Tokyo Metropolitan Institute of Medical Science Rinshoken in 2007. Exon 3 of the Capn9 gene was replaced with a neomycin resistant cassette. C57BL/6 background.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References
Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense.
Hata S, Abe M, Suzuki H, Kitamura F, Toyama-Sorimachi N, Abe K, Sakimura K, Sorimachi H
PLoS Genet., 6, e1001040 (2010). 20686710

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Capn9calpain 98Capn9targeted mutation 1, Hiroyuki Sorimachi
Frtyeast FRT (flippase recombination target) site8Frt
Frtyeast FRT (flippase recombination target) site8Frt
loxPphage P1 loxP8loxP
loxPphage P1 loxP8loxP
neoneomycin resistance gene (E. coli)8
poly ApolyA8poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • digestive/alimentary phenotype(MP:0005381)

  • increased susceptibility to injury(MP:0005165)
    • Detailed phenotype data

    Ordering Information

    Donor DNAbacteriophage P1 LoxP sites, yeast FRT sites, mouse pgk promoter, E. coli Neomycin resistance gene, mouse pgk polyA signal, mouse Capn9 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
    1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.
    PLoS Genet. 2010 6(7):e1001040.
    2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    3) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research which is premised on publication.
    4) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    5) In presenting the research results obtained by use of the BIOLOGICAL RESOURCE for the first time in a form of international publications, it is required that the RECIPIENT contacts the DEPOSITOR for agreement and that the DEPOSITOR and its colleagues are included as co-authors, who are designated by the DEPOSITOR.
    DepositorHiroyuki Sorimachi (Tokyo Metropolitan Institute of Medical Science)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data