RBRC04790

Information1

Image
BRC No.RBRC04790
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Capn9<tm1Hiso>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Drs. Shoji Hata and Hiroyuki Sorimachi, The Tokyo Metropolitan Institute of Medical Science Rinshoken in 2007. Exon 3 of the Capn9 gene was replaced with a neomycin resistant cassette. C57BL/6 background.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
ReferencesPLoS Genet., 6, e1001040 (2010). 20686710

Health Report

Examination Date / Room / Rack2011/09/05Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Capn9calpain 98Capn9<tm1Hiso>targeted mutation 1, Hiroyuki Sorimachi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ApolyA8poly A

Information2

Donor DNAE. coli Neomycin resistance gene, bacteriophage P1 LoxP sequence, yeast FRT sequence, mouse, pgk promoter, mouse pgk polyA signal
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.
PLoS Genet. 2010 6(7):e1001040.
2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
3) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research which is premised on publication.
4) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
5) In presenting the research results obtained by use of the BIOLOGICAL RESOURCE for the first time in a form of international publications, it is required that the RECIPIENT contacts the DEPOSITOR for agreement and that the DEPOSITOR and its colleagues are included as co-authors, who are designated by the DEPOSITOR.
DepositorHiroyuki Sorimachi (Tokyo Metropolitan Institute of Medical Science)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data