Strain Data Sheet

RBRC04774

Information1

Image
BRC No.RBRC04774
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnmt1<tm1Okan>/Rbrc
Former Common nameDnmt1(Cys1229Ser), Dnmt1<tm1Okan> (Acc.No.CDB0500K)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Dr. Masaki Okano, Center for Developmental Biology RIKEN. A point mutation in exon 32 of the Dnmt1 gene introduced an amino acid substitution Cys1229Ser. An IRES-betageo cassette was inserted into an upstream of the point mutation and was removed by cre-mediated recombination.
Strain descriptionDNA methltransferase 1 gene C1229S knock-in mice. A point mutation in exon 32 of the Dnmt1 gene introduced an amino acid substitution Cys1229Ser. The mutant allele that expresses a C1229S mutated protein lacks catalytic activity. The C1229S mutant mice show developmental arrest, near-complete loss of DNA methylation, and an altered distribution of repressive chromatin markers. Embryonic lethal (E8.0-8.5).
Colony maintenance
ReferencesMol. Cell. Biol., 27, 8243-8258 (2007). 17893328

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnmt1DNA methyltransferase (cytosine-5) 19Dnmt1<tm1Okan>targeted mutation 1, Masaki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Information2

Donor DNAphage P1 loxP site, mouse Dnmt1 genomic DNA
Research applicationCre/loxP system
Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No.CDB0500K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. Mol Cell Biol. 2007 27(23):8243-58. (3) The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes.
DepositorMasaki Okano (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jun 2011
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data