RBRC04773

Information1

Image
BRC No.RBRC04773
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR;B6C3-Tg(CAG-cat,-HTF/Nanos2)7
Former Common nameHTF-nanos2 #7
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Atsushi Suzuki, Interdisciplinary Research Center, Yokohama National University in 2009.
Strain description
Colony maintenance
ReferencesScience, 325, 1394-1398 (2009). 19745153
Genes Dev., 22, 430-435 (2008). 18281459

Health Report

Examination Date / Room / Rack2012/01/10Room:2-3Rack:C
2011/11/07Room:2-3Rack:C
2011/09/05Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nanos2nanos homolog 2 (Drosophila)UNNanos2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
TEVTobacco Etch Virus (TEV) proteaseUNTEV

Gene symbolGene nameChr.Allele symbolAllele namePromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), E. coli chloramphenicol acetyltransferase, mouse Nanos2 gene cDNA, human influenza virus Ha (hemagglutinin) tag cDNA, Tobacco Etch Virus (TEV) protease
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from DEPOSITOR and Prof. Yumiko Saga, National Institute of Genetics. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and /or the DEVELOPER's names as co-authors.
DepositorAtsushi Suzuki (Yokohama National University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data