Strain Data Sheet

RBRC04773

Strain Information

Image
BRC No.RBRC04773
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR;B6C3-Tg(CAG-cat,-HTF/Nanos2)7
Former Common nameHTF-nanos2 #7
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Atsushi Suzuki, Interdisciplinary Research Center, Yokohama National University in 2009.
Strain description
Colony maintenance
References
Nanos2 suppresses meiosis and promotes male germ cell differentiation.
Suzuki A, Saga Y
Genes Dev., 22, 430-435 (2008). 18281459

The RNA-binding protein NANOS2 is required to maintain murine spermatogonial stem cells.
Sada A, Suzuki A, Suzuki H, Saga Y
Science, 325, 1394-1398 (2009). 19745153

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAGFLAG tag (synthetic)UNFLAG
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA
Nanos2nanos homolog 2 (Drosophila)UNNanos2
TEVTobacco Etch Virus (TEV) proteaseUNTEV
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
loxPphage P1 loxPUNloxP
loxPphage P1 loxPUNloxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), E. coli chloramphenicol acetyltransferase, mouse Nanos2 gene cDNA, human influenza virus Ha (hemagglutinin) tag cDNA, Tobacco Etch Virus (TEV) protease
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from DEPOSITOR and Prof. Yumiko Saga, National Institute of Genetics. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and /or the DEVELOPER's names as co-authors.
    DepositorAtsushi Suzuki (Yokohama National University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data