RBRC04746

Information1

Image
BRC No.RBRC04746
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(aMHC-GCaMP2)53Bsi
Former Common nameaMHC-GCaMP2#53
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Thomas Knöpfel, RIKEN Brain Science Institute.
Strain descriptionTransgene composed of regulatory sequences of mouse aMHC promoter and rat Kv3.1 (Kcnc1) and the gene for the calcium sensor GCaMP2. GCaMP2: Ca2+-sensing GCaMP2 protein consist of M13 segment of myosin light chain kinase from chicken, Green Fluorescent Protein (GFP) from jelly fish, and calmodulin from rat (Eur J Neurosci. 2005 Aug;22(3):627-35). There are 5 lines of aMHCA-GcaMP2 tansgenic mice (RBRC04743, RBRC04744, RBRC04745, RBRC04746, RBRC04747). The heart condition of the aMHCA-GcaMP2 mice differs from line to line. Dependent on the expression level, some lines exhibit some heart hypertrophy. Line #4 (RBRC04743): Expression level is low to medium. Homozygous mice are fertile. Line #14 (RBRC04744): Expression level is medium. Hypertrophic heart. Homozygous mice are fertile. Line #29 (RBRC04745): Expression might be mosaic (varying penetrance?). Homozygous mice are fertile. Line #53 (RBRC04746): Strong expression. Viability and reproductive capacity of homozygote is unknown. Line #60 (RBRC04747): Very strong expression. So far no surviving (2 months <) (homozygote).
Colony maintenanceCarrier x NoncarrierThe different lines have different levels of GCamP2 expression. Dependent on the expression level, some lines exhibit some heart hypertrophy. These mice are fertile but might not reach ages over 3-4 months.
ReferencesEur. J. Neurosci., 22, 627-635 (2005). 16101744

Health Report

Examination Date / Room / Rack2013/04/15Room:3-CRack:C
2013/02/18Room:3-CRack:C
2012/12/10Room:3-CRack:C
2012/11/28Room:3-CRack:C
2012/11/26Room:3-CRack:C
2012/10/15Room:3-CRack:C
2012/10/15Room:3-CRack:C
2012/08/27Room:3-CRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GCaMP2RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP2mouse aMHC promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAmouse aMHC promoter, GCaMP2(RSET tag, chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Simian virus SV40 polyA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsBEFORE the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
a) The RECIPIENT shall acquire a prior written consent from the DEPOSITOR using the Approval form.
b) The RECIPIENT shall acquire and a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
c) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested, Eur J Neurosci. 2005 Aug;22(3):627-35.
d) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
AFTER the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
a) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested, (will be announced).
b) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorThomas Knöepfel (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Contact Information

BRC mice in Publications

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