Strain Data Sheet

RBRC04740

Strain Information

Image
BRC No.RBRC04740
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.B6CB-Ltb4r1<tm1Tksh>
Former Common nameBALB-BLT1 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cCrSlc
Appearance
Strain developmentDeveloped by Dr. Takao Shimizu, Faculty of Medicine, University of Tokyo in 2005. A lacZ-neo was inserted exon 2 of the Ltb4r1 gene (Expression of lacZ was not detected). The mutant mice were backcrossed to BALB/c.
Strain description
Colony maintenance
References
A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis.
T Yokomizo, T Izumi, K Chang, Y Takuwa, T Shimizu
Nature, 387, 620-624 (1997). 9177352

Absence of leukotriene B4 receptor 1 confers resistance to airway hyperresponsiveness and Th2-type immune responses.
Kan Terawaki, Takehiko Yokomizo, Takahide Nagase, Akiko Toda, Masahiko Taniguchi, Kohei Hashizume, Takeshi Yagi, Takao Shimizu
J. Immunol., 175, 4217-4225 (2005). 16177061

A distinctive role of the leukotriene B4 receptor BLT1 in osteoclastic activity during bone loss.
Hisako Hikiji, Satoshi Ishii, Takehiko Yokomizo, Tsuyoshi Takato, Takao Shimizu
Proc. Natl. Acad. Sci. USA, 106, 21294-21299 (2009). 19965376

The leukotriene B4 receptor, BLT1, is required for the induction of experimental autoimmune encephalomyelitis.
Yasuyuki Kihara, Takehiko Yokomizo, Akiko Kunita, Yasuyuki Morishita, Masashi Fukayama, Satoshi Ishii, Takao Shimizu
Biochem. Biophys. Res. Commun., 394, 673-678 (2010). 20226760

The LTB4-BLT1 axis mediates neutrophil infiltration and secondary injury in experimental spinal cord injury.
Hirokazu Saiwai, Yasuyuki Ohkawa, Hisakata Yamada, Hiromi Kumamaru, Akihito Harada, Hideyuki Okano, Takehiko Yokomizo, Yukihide Iwamoto, Seiji Okada
Am. J. Pathol., 176, 2352-2366 (2010). 20304963

Attenuated Th1 induction by dendritic cells from mice deficient in the leukotriene B4 receptor 1.
Akiko Toda, Kan Terawaki, Soh Yamazaki, Kazuko Saeki, Takao Shimizu, Takehiko Yokomizo
Biochimie, 92, 682-691 (2010). 20004699

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ltb4r1
MGI:1309472		leukotriene B4 receptor 114Ltb4r1<tm1Tksh>
MGI:3701260		targeted mutation 1, Takao Shimizu

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cornea wound healing (MP:0020861)

  • abnormal cytokine secretion (MP:0003009)

  • abnormal granulocyte physiology (MP:0002462)

  • abnormal hormone level (MP:0003953)

  • abnormal mast cell physiology (MP:0002423)
    • more 12 phenotypes
  • abnormal respiratory system physiology (MP:0002133)

  • abnormal sensitization (MP:0011618)

  • decreased IgE level (MP:0002492)

  • decreased airway responsiveness (MP:0002335)

  • decreased cell proliferation (MP:0000352)

  • decreased eosinophil cell number (MP:0005012)

  • decreased susceptibility to type I hypersensitivity reaction (MP:0005597)

  • delayed wound healing (MP:0002908)

  • edema (MP:0001785)

  • increased susceptibility to induced colitis (MP:0008537)

  • increased thigmotaxis (MP:0002797)

  • lung inflammation (MP:0001861)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Ltb4r1 genomic DNA, E.coli beta-galactosidase, Mouse phophoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, Mouse polyA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 175, 4217-4225 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTakao Shimizu (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data