Strain Data Sheet

RBRC04740

Strain Information

Image
BRC No.RBRC04740
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.B6CB-Ltb4r1<tm1Tksh>
Former Common nameBALB-BLT1 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cCrSlc
Appearance
Strain developmentDeveloped by Dr. Takao Shimizu, Faculty of Medicine, University of Tokyo in 2005. A lacZ-neo was inserted exon 2 of the Ltb4r1 gene (Expression of lacZ was not detected). The mutant mice were backcrossed to BALB/c.
Strain description
Colony maintenance
References
Absence of leukotriene B4 receptor 1 confers resistance to airway hyperresponsiveness and Th2-type immune responses.
Terawaki K, Yokomizo T, Nagase T, Toda A, Taniguchi M, Hashizume K, Yagi T, Shimizu T
J. Immunol., 175, 4217-4225 (2005). 16177061

The LTB4-BLT1 axis mediates neutrophil infiltration and secondary injury in experimental spinal cord injury.
Saiwai H, Ohkawa Y, Yamada H, Kumamaru H, Harada A, Okano H, Yokomizo T, Iwamoto Y, Okada S
Am. J. Pathol., 176, 2352-2366 (2010). 20304963

A distinctive role of the leukotriene B4 receptor BLT1 in osteoclastic activity during bone loss.
Hikiji H, Ishii S, Yokomizo T, Takato T, Shimizu T
Proc. Natl. Acad. Sci. USA, 106, 21294-21299 (2009). 19965376

Attenuated Th1 induction by dendritic cells from mice deficient in the leukotriene B4 receptor 1.
Toda A, Terawaki K, Yamazaki S, Saeki K, Shimizu T, Yokomizo T
Biochimie, 92, 682-691 (2010). 20004699

A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis.
Yokomizo T, Izumi T, Chang K, Takuwa Y, Shimizu T
Nature, 387, 620-624 (1997). 9177352

The leukotriene B4 receptor, BLT1, is required for the induction of experimental autoimmune encephalomyelitis.
Kihara Y, Yokomizo T, Kunita A, Morishita Y, Fukayama M, Ishii S, Shimizu T
Biochem. Biophys. Res. Commun., 394, 673-678 (2010). 20226760

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ltb4r1leukotriene B4 receptor 114Ltb4r1targeted mutation 1, Takao Shimizu

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cornea wound healing(MP:0020861)

  • abnormal cytokine secretion(MP:0003009)

  • abnormal granulocyte physiology(MP:0002462)

  • abnormal hormone level(MP:0003953)

  • abnormal mast cell physiology(MP:0002423)
    • more 12 phenotypes
  • abnormal respiratory system physiology(MP:0002133)

  • abnormal sensitization(MP:0011618)

  • decreased IgE level(MP:0002492)

  • decreased airway responsiveness(MP:0002335)

  • decreased cell proliferation(MP:0000352)

  • decreased eosinophil cell number(MP:0005012)

  • decreased susceptibility to type I hypersensitivity reaction(MP:0005597)

  • delayed wound healing(MP:0002908)

  • edema(MP:0001785)

  • increased susceptibility to induced colitis(MP:0008537)

  • increased thigmotaxis(MP:0002797)

  • lung inflammation(MP:0001861)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Ltb4r1 genomic DNA, E.coli beta-galactosidase, Mouse phophoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, Mouse polyA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 175, 4217-4225 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTakao Shimizu (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data