RBRC04737

Information1

Image
BRC No.RBRC04737
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Nmu<tm1Mko> Nms<tm1Mko>
Former Common nameNMU/NMS-dKO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masayasu Kojima, Institute of Life Science, Kurume University in 2000. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6.
Strain descriptionNmu (Neuromedin U) and Nmus (Neuromedin S) gene double knock-out mice. Nmu KO: Exon 9 of the Nmu gene was replaced with a PGK-neo cassette. Homozygous mutant mice show a increased body weight. Nms KO: Exon 8 of the Nms gene was replaced with a neo cassette. Homozygous mutant mice show no obvious abnormality. Nmu gene knockout mice (RBRC04549), Nms gene knockout mice (RBRC04550).
Colony maintenanceNmu: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]; Nms: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNat. Med., 10, 1067-1073 (2004). 15448684
J. Exp. Med., 202, 217-224 (2005). 16009716

Health Report

Examination Date / Room / Rack2015/10/19Room:3-CRack:C
2015/08/17Room:3-CRack:C
2015/06/15Room:3-CRack:C
2015/05/27Room:3-CRack:C
2015/05/25Room:3-CRack:C
2015/04/20Room:3-CRack:C
2015/02/16Room:3-CRack:C
2014/12/15Room:3-CRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nmsneuromedin S1Nms<tm1Mko>targeted mutation 1, Masayasu Kojima

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nmuneuromedin U5Nmu<tm1Mko>targeted mutation 1, Masayasu Kojima

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nmu genomic DNA, mouse Nms genomic DNA
Research applicationMetabolism Research
Specific Term and ConditionsBecause no paper has been published on neuromedin S knockout mice, the RECIPIENT is requested to contact the DEPOSITOR in publishing the research results obtained by use of the BIOLOGICAL RESOURCE.
DepositorMsayasu Kojima (Kurume University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data