Strain Data Sheet

RBRC04737

Strain Information

Image
BRC No.RBRC04737
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Nmu<tm1Mko> Nms<tm1Mko>
Former Common nameNMU/NMS-dKO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masayasu Kojima, Institute of Life Science, Kurume University in 2000. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6.
Strain descriptionNmu (Neuromedin U) and Nmus (Neuromedin S) gene double knock-out mice. Nmu KO: Exon 9 of the Nmu gene was replaced with a PGK-neo cassette. Homozygous mutant mice show a increased body weight. Nms KO: Exon 8 of the Nms gene was replaced with a neo cassette. Homozygous mutant mice show no obvious abnormality. Nmu gene knockout mice (RBRC04549), Nms gene knockout mice (RBRC04550).
Colony maintenanceNmu: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]; Nms: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Neuromedin U has a novel anorexigenic effect independent of the leptin signaling pathway.
Hanada R, Teranishi H, Pearson J T, Kurokawa M, Hosoda H, Fukushima N, Fukue Y, Serino R, Fujihara H, Ueta Y, Ikawa M, Okabe M, Murakami N, Shirai M, Yoshimatsu H, Kangawa K, Kojima M
Nat. Med., 10, 1067-1073 (2004). 15448684

The neuropeptide neuromedin U promotes inflammation by direct activation of mast cells.
Moriyama M, Sato T, Inoue H, Fukuyama S, Teranishi H, Kangawa K, Kano T, Yoshimura A, Kojima M
J. Exp. Med., 202, 217-224 (2005). 16009716

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nmsneuromedin S1Nmstargeted mutation 1, Masayasu Kojima
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)
Nmuneuromedin U5Nmutargeted mutation 1, Masayasu Kojima
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circadian behavior(MP:0020467)

  • abnormal glucose homeostasis(MP:0002078)

  • abnormal lipid homeostasis(MP:0002118)

  • decreased circulating corticosterone level(MP:0002665)

  • decreased locomotor activity(MP:0001402)
    • more 12 phenotypes
  • decreased oxygen consumption(MP:0005290)

  • hepatic steatosis(MP:0002628)

  • hyperglycemia(MP:0001559)

  • impaired adaptive thermogenesis(MP:0011049)

  • increased circulating cholesterol level(MP:0005178)

  • increased circulating free fatty acids level(MP:0001554)

  • increased circulating insulin level(MP:0002079)

  • increased circulating leptin level(MP:0005669)

  • increased fat cell size(MP:0006094)

  • increased triglyceride level(MP:0005317)

  • obese(MP:0001261)

  • polyphagia(MP:0001433)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nmu genomic DNA, mouse Nms genomic DNA
    Research applicationMetabolism Research
    Specific Term and ConditionsBecause no paper has been published on neuromedin S knockout mice, the RECIPIENT is requested to contact the DEPOSITOR in publishing the research results obtained by use of the BIOLOGICAL RESOURCE.
    DepositorMsayasu Kojima (Kurume University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data