RBRC04720

Information1

Image
BRC No.RBRC04720
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Rap1a<tm1Morz> Rap1b<tm1Morz>
Former Common nameB6-Rap1a/Rap1b floxed
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentNational Institute of Mental Health・Alexei Morozov.Developed by Alexei Morozov, National Institute of Mental Health. 129 ES cell derived. The mice were crossed to C57BL/6.
Strain descriptionRap1a and Rap1b floxed mice. Exons 2-3 of Rap1a gene and exon 1 of Rap1b gene were flanked by loxP sites.
Colony maintenanceRap1a : Homozygote x Homozygote ; Rap1b : Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
ReferencesJ. Neurosci., 28, 2089-2098 (2008). 18305243

Health Report

Examination Date / Room / Rack2014/06/16Room:3-ARack:L
2014/04/21Room:3-ARack:L
2014/02/18Room:3-ARack:L
2013/12/16Room:3-ARack:L
2013/10/21Room:3-ARack:L
2013/08/26Room:3-ARack:L
2013/06/17Room:3-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rap1bRAS-related protein-1b10Rap1b<tm1Morz>targeted mutation 1, Alexei Morozov

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rap1aRAS-related protein-1a3Rap1a<tm1Morz>targeted mutation 1, Alexei Morozov

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAmouse Rapla genomic DNA, mouse Raplb genomic DNA, herpers simplex thymidine kinase promoter, E. coli Neomycin resistance gene, Bacteriophage P1 loxP site
Research applicationCre/loxP system
Specific Term and Conditionsa) In all RECIPIENT publications related to the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested:
J Neurosci. 2008 Feb 27;28(9):2089-98.Enhanced cortico-amygdala efficacy and suppressed fear in absence of Rap1. Pan BX, Vautier F, Ito W, Bolshakov VY, Morozov A.
b) In all publications or presentations of any research results obtained by use of the BIOLOGICAL RESOURCE, RECIPIENT will acknowledge the DEPOSITOR as the source of the BIOLOGICAL RESOURCE.
c) Use of the BIOLOGICAL RESOURCE for commercial purposes will require a license agreement between NIH and RECIPIENT. Information can be obtained at http://www.ott.nih.gov.
d) Prior to filing an application for a patent, or intellectual property or other rights based on the use of the BIOLOGICAL RESOURCE, RECIPIENT will first notify the DEPOSITOR.
e) RECIPIENT agrees not to claim, infer, or imply endorsement by the DEPOSITOR.
f) Unless prohibited by law from doing so, RECIPIENT agrees to hold the DEPOSITOR harmless and to indemnify the DEPOSITOR for all liabilities, demands, damages, expenses and losses arising out of RECIPIENT 's use for any purpose of the BIOLOGICAL RESOURCE.
g) This BIOLOGICAL RESOURCE is provided as a service to the research community. IT IS BEING SUPPLIED TO RECIPIENT WITH NO WARRANTIES, EXPRESS OR IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. RECIPIENT acknowledges that DEPOSITOR makes no representations that the use of the BIOLOGICAL RESOURCE will not infringe any patent or proprietary rights of third parties.
DepositorAlexei Morozov (National Institutes of Health)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Nagai T, Nakamuta S, Kuroda K, Nakauchi S, Nishioka T, Takano T, Zhang X, Tsuboi D, Funahashi Y, Nakano T, Yoshimoto J, Kobayashi K, Uchigashima M, Watanabe M, Miura M, Nishi A, Kobayashi K, Yamada K, Amano M, Kaibuchi K.
Phosphoproteomics of the Dopamine Pathway Enables Discovery of Rap1 Activation as a Reward Signal In Vivo.
Neuron 89(3) 550-65. (2016) 26804993