RBRC04718

Information1

Image
BRC No.RBRC04718
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Cluap1<tm1Hmd>
Former Common nameQilin (flox/+)
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Yanick Botlde and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6/129. Correctly targeted ES cells were aggregated with ICR molura. The resulting chimeric animals were crossed to C57BL/6 and then crossed to CAG-FLPe mice.
Strain descriptionCluap1 (Qilin) gene floxed mice. Exons 1 to 3 were flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesDev Biol., 381(1), 203-212 (2013). 23742838

Health Report

Examination Date / Room / Rack2012/09/24Room:4-ARack:F
2012/07/23Room:4-ARack:F
2012/05/21Room:4-ARack:F
2012/03/26Room:4-ARack:F
2012/01/23Room:4-ARack:F
2011/12/14Room:4-ARack:F
2011/12/12Room:4-ARack:F
2011/12/12Room:4-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cluap1clusterin associated protein 116Cluap1<tm1.1Hmd>targeted mutation 1.1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP16loxP

Information2

Donor DNAbacteriophage由来 loxP配列, yeast由来 frt配列, chicken/rabbit/Cytomegalovirus由来 CAGプロモーター, yeast由来 flpe遺伝子
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data