RBRC04714

Information1

Image
BRC No.RBRC04714
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Spesp1<tm1Osb>
Former Common nameSpesp1 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Drs.Yoshitaka Fujihara and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2006.
Strain description
Colony maintenanceHeterozygote X Wild-type [C57BL/6NJcl]
ReferencesJ. Cell. Sci., 123, 1531-1536 (2010). 20375058

Health Report

Examination Date / Room / Rack2012/10/15Room:3-CRack:F
2012/08/27Room:3-CRack:F
2012/06/18Room:3-CRack:F
2012/04/16Room:3-CRack:F
2012/02/20Room:3-CRack:F
2011/12/12Room:3-CRack:F
2011/10/17Room:3-CRack:F
2011/08/29Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Spesp1sperm equatorial segment protein 19Spesp1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9

Information2

Donor DNAmouse Spesp1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter) and polyA signal, P1 phage loxP, yeast FRT (flipase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 123, 1531-1536 (2010).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Nakata H, Wakayama T, Asano T, Nishiuchi T, Iseki S.
Identification of sperm equatorial segment protein 1 in the acrosome as the primary binding target of peanut agglutinin (PNA) in the mouse testis.
Histochem. Cell Biol. 147 27-38(2017) 27539077