Strain Data Sheet

RBRC04708

Strain Information

Image
BRC No.RBRC04708
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameFVB.Cg-Tg(Slc6a5-EGFP)13Uze
Former Common nameGlyT2GfpBL13
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Jean-Marc Fritschy, University of Zurich. Deposited by Thomas Knoepfel, RIKEN Brain Science Institute. BAC clone 365E4 from the mouse C57BL/6 BAC library RPCI-23 (Research Genetics Inc., Huntsville, AL) containing the GlyT2 gene. Mixed background (FVB mixed).
Strain descriptionGlyT2-GFP transgenic mice. Transgene composed of BAC containing the GlyT2 (Slc6A5) gene with EGFP. Expression of GFP under the GlyT2 promoter. Homozygous mice are viable but infertile.
Colony maintenanceCarrier x Noncarrier
References
Glycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome transgenic mice.
Zeilhofer H U, Studler B, Arabadzisz D, Schweizer C, Ahmadi S, Layh B, Bosl M R, Fritschy J M
J. Comp. Neurol., 482, 123-141 (2005). 15611994

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse GlyT2 promoter
  • hyperekplexia 3(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse GlyT2 promoter, Jellyfish Green Fluorescent Protein cDNA, Bovine growth hormone polyadenylation (poly-A) signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorThomas Knöepfel (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data