RBRC04707

Information1

Image
BRC No.RBRC04707
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Kcnc1-EYFP)27Bsi Gad1<tm1Tama>
Former Common nameKv3.GYW
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Thomas Knoepfel, RIKEN Brain Science Institute.
Strain descriptionKcnc1-YFP transgenic and GAD67 GFP knock-in double mice. Transgene composed of 5kB 5’ regulatory sequence of rat Kv3.1 potassium channel driving expression of a yellow variant of jelly fish green fluorescent protein. Knock-in of GFP into the ATG in exon 1 of GAD67 gene. Bright yellow and green fluorescence of specific cell types. GAD67 GFP homozygous mice are infertile.
Colony maintenanceTg : Carrier x Carrier; KO : Heterozygote x Wild-type
ReferencesJ. Comp. Neurol., 467, 60-79 (2003). 14574680
Eur. J. Neurosci., 15, 40-50 (2002). 11860505

Health Report

Examination Date / Room / Rack2014/06/02Room:3-7Rack:B
2014/04/07Room:3-7Rack:B
2014/02/03Room:3-7Rack:B
2013/12/02Room:3-7Rack:B
2013/10/07Room:3-7Rack:B
2013/08/05Room:3-7Rack:B
2013/06/03Room:3-7Rack:B
2013/04/01Room:3-7Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gad1glutamate decarboxylase 12Gad1<tm1Tama>targeted mutation 1, Nobuaki Tamamaki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFPrat Kcnc1 promoter

Information2

Donor DNArat Kv3. 1 (Kcnc1) genomic DNA, Jellyfish EYFP cDNA, Simian virus SV40 polyA, mouse GAD67 genimic DNA, Jellyfish GFP cDNA, Phage P1 loxP, [Mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene ]
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsBEFORE the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
a. The RECIPIENT shall acquire a prior written consent from the DEPOSITOR using the Approval form.
b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
c. The RECIPIENT shall acquire and a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
d. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested, Eur J Neurosci. 2002 15(1):40-50., J Comp Neurol. 2003 467(1):60-79.
AFTER the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
a. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested, (will be announced).
b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorThomas Knöepfel (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Contact Information

BRC mice in Publications

No Data