Strain Data Sheet

RBRC04707

Strain Information

Image
BRC No.RBRC04707
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Kcnc1-EYFP)27Bsi Gad1<tm1Tama>
Former Common nameKv3.GYW
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Thomas Knoepfel, RIKEN Brain Science Institute.
Strain descriptionKcnc1-YFP transgenic and GAD67 GFP knock-in double mice. Transgene composed of 5kB 5’ regulatory sequence of rat Kv3.1 potassium channel driving expression of a yellow variant of jelly fish green fluorescent protein. Knock-in of GFP into the ATG in exon 1 of GAD67 gene. Bright yellow and green fluorescence of specific cell types. GAD67 GFP homozygous mice are infertile.
Colony maintenanceTg : Carrier x Carrier; KO : Heterozygote x Wild-type
References
Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse.
Tamamaki N, Yanagawa Y, Tomioka R, Miyazaki J, Obata K, Kaneko T
J. Comp. Neurol., 467, 60-79 (2003). 14574680

Transgenic mice expressing a pH and Cl- sensing yellow-fluorescent protein under the control of a potassium channel promoter.
Metzger F, Repunte-Canonigo V, Matsushita S, Akemann W, Diez-Garcia J, Ho C S, Iwasato T, Grandes P, Itohara S, Joho R H, Knopfel T
Eur. J. Neurosci., 15, 40-50 (2002). 11860505

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)2
  • developmental and epileptic encephalopathy 89(MedGEN)

  • progressive myoclonic epilepsy type 7(MedGEN)
    • Gad1glutamate decarboxylase 12Gad1targeted mutation 1, Nobuaki Tamamaki
  • developmental and epileptic encephalopathy 89(MedGEN)

  • progressive myoclonic epilepsy type 7(MedGEN)
    • loxPphage P1 loxP2loxP
  • developmental and epileptic encephalopathy 89(MedGEN)

  • progressive myoclonic epilepsy type 7(MedGEN)
    • EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFPrat Kcnc1 promoter
  • developmental and epileptic encephalopathy 89(MedGEN)

  • progressive myoclonic epilepsy type 7(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal involuntary movement(MP:0003492)

  • abnormal nervous system electrophysiology(MP:0002272)
    • Detailed phenotype data

    Ordering Information

    Donor DNArat Kv3. 1 (Kcnc1) genomic DNA, Jellyfish EYFP cDNA, Simian virus SV40 polyA, mouse GAD67 genimic DNA, Jellyfish GFP cDNA, Phage P1 loxP, [Mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene ]
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsBEFORE the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
    a. The RECIPIENT shall acquire a prior written consent from the DEPOSITOR using the Approval form.
    b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    c. The RECIPIENT shall acquire and a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    d. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by the DEPOSITOR is requested, Eur J Neurosci. 2002 15(1):40-50., J Comp Neurol. 2003 467(1):60-79.
    AFTER the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
    a. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested, (will be announced).
    b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorThomas Knöepfel (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data