Strain Data Sheet

RBRC04706

Strain Information

Image
BRC No.RBRC04706
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(aMHC-VSFP2.3)123Bsi
Former Common name
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Thomas Knoepfel, RIKEN Brain Science Institute. C57BL/6, DBA/2 and ICR mixed background.
Strain descriptionTransgene composed of regulatory sequences of mouse aMHC promoter and the gene for the voltage sensor VSFP2.3. Tg homozygots mating shows poor reproduction.
Colony maintenanceCarrier x Noncarrier
References
Transgenic mice expressing a pH and Cl- sensing yellow-fluorescent protein under the control of a potassium channel promoter.
Metzger F, Repunte-Canonigo V, Matsushita S, Akemann W, Diez-Garcia J, Ho C S, Iwasato T, Grandes P, Itohara S, Joho R H, Knopfel T
Eur. J. Neurosci., 15(1):40-50 (2002). 11860505

Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
Lundby A, Mutoh H, Dimitrov D, Akemann W, Knopfel T
PLoS One, 3, e2514 (2008). 18575613

Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins.
Akemann W, Mutoh H, Perron A, Rossier J, Knopfel T
Nat. Methods., 7, 643-649 (2010). 20622860

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
VSFP2.3voltage-sensitive Fluorescent Protein 2.3, EYFP (Aequorea victoria)UNVSFP2.3mouse aMHC promoter
  • atrial septal defect 3(MedGEN)

  • dilated cardiomyopathy 1EE(DisGeNET, MedGEN)

  • hypertrophic cardiomyopathy 14(DisGeNET, MedGEN)
    • more 2 Diseases
  • hypertrophic cardiomyopathy 1(MedGEN)

  • sick sinus syndrome 3, susceptibility to(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse aMHC promoter, Ciona intestinalis calcium sensor VSFP2. 3 cDNA
    Research application
    Specific Term and ConditionsBEFORE the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
    a. The RECIPIENT shall acquire a prior written consent from the DEPOSITOR using the Approval form.
    b. The RECIPIENT shall acquire and a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
    c. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    d. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested, Eur J Neurosci. 2002 15(1):40-50.
    AFTER the first publication on the BIOLOGICAL RESOURCE by the DEPOSITOR:
    a. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested, (will be announced).
    b. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorThomas Knöepfel (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information
    Genotyping protocol -PCR-
    Mouse of the Month Mar 2011

    BRC mice in Publications

    No Data