RBRC04704

Information1

Image
BRC No.RBRC04704
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Pih1d3<tm1.1Hmd>
Former Common nameTwister (+/-)
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2006. The Pih1d3 gene was removed by crossing with CAG-FLPe and CAG-cre mice.
Strain descriptionPih1d3 (Stp/Twister) gene knockout mice. Homozygous mutant males are infertile (females are fertile).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ. Cell Biol., 204, 203-213 (2014). 24421334

Health Report

Examination Date / Room / Rack2012/12/03Room:3-8Rack:C
2012/10/01Room:3-8Rack:C
2012/09/19Room:3-8Rack:C
2012/09/18Room:3-8Rack:C
2012/08/06Room:3-8Rack:C
2012/06/04Room:3-8Rack:C
2012/04/02Room:3-8Rack:C
2012/02/06Room:3-8Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pih1d3PIH1 domain containing 31Pih1d3<tm1.2Hmd>targeted mutation 1.2, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP1loxP

Information2

Donor DNAbacteriophage P1 loxP site, mouse Pih1d3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data