Strain Data Sheet

RBRC04704

Strain Information

Image
BRC No.RBRC04704
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Pih1d3<tm1.1Hmd>
Former Common nameTwister (+/-)
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2006. The Pih1d3 gene was removed by crossing with CAG-FLPe and CAG-cre mice.
Strain descriptionPih1d3 (Stp/Twister) gene knockout mice. Homozygous mutant males are infertile (females are fertile).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.
Dong F, Shinohara K, Botilde Y, Nabeshima R, Asai Y, Fukumoto A, Hasegawa T, Matsuo M, Takeda H, Shiratori H, Nakamura T, Hamada H
J. Cell Biol., 204, 203-213 (2014). 24421334

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pih1d3PIH1 domain containing 31Pih1d3targeted mutation 1.2, Hiroshi Hamada
  • ciliary dyskinesia, primary, 36, X-linked(MedGEN)
    • loxPphage P1 loxP1loxP
  • ciliary dyskinesia, primary, 36, X-linked(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal male reproductive system physiology(MP:0003698)

  • abnormal reproductive system morphology(MP:0002160)

  • abnormal sperm axoneme morphology(MP:0009838)

  • abnormal sperm flagellum morphology(MP:0008892)

  • asthenozoospermia(MP:0002675)
    • more 7 phenotypes
  • growth/size/body region phenotype(MP:0005378)

  • male infertility(MP:0001925)

  • renal/urinary system phenotype(MP:0005367)

  • reproductive system phenotype(MP:0005389)

  • respiratory system phenotype(MP:0005388)

  • short sperm flagellum(MP:0009239)

  • thin sperm flagellum(MP:0009242)
    • Detailed phenotype data

    Ordering Information

    Donor DNAbacteriophage P1 loxP site, mouse Pih1d3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Biol., 204, 203-213 (2014).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data