RBRC04703

Information1

Image
BRC No.RBRC04703
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Pih1d3<tm1Hmd>
Former Common nameTwister (flox/+)
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2006. A PGK-neo cassette was removed by crossing with CAG-FLPe mice.
Strain descriptionPih1d3 (Stp/Twister) gene floxed mice. Expression of Pih1d3 is detected by X-gal staining.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ Cell Biol., 204, 203-213 (2014). 24421334

Health Report

Examination Date / Room / Rack2012/11/26Room:4-ARack:E
2012/09/24Room:4-ARack:E
2012/07/23Room:4-ARack:E
2012/05/21Room:4-ARack:E
2012/03/26Room:4-ARack:E
2012/01/23Room:4-ARack:E
2011/11/28Room:4-ARack:E
2011/10/19Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pih1d3PIH1 domain containing 31Pih1d3<tm1Hmd>targeted mutation 1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site1Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
myc-tagmyc-epitope tag1myc-tag

Information2

Donor DNAphage P1 loxP, yeast FRT (flipase recombination target) site, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, Myc-tag, mouse Pih1d3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data