Strain Data Sheet

RBRC04702

Strain Information

Image
BRC No.RBRC04702
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129B6-Edem1<tm3Hos>/1H
Former Common nameEDEM1 null
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Nobuko Hosokawa, Institute for Frontier Medical Sciences, Kyoto University in 2005.
Strain description
Colony maintenance
References
EDEM1 accelerates the trimming of alpha1,2-linked mannose on the C branch of N-glycans.
Hosokawa N, Tremblay L O, Sleno B, Kamiya Y, Wada I, Nagata K, Kato K, Herscovics A
Glycobiology, 20, 567-575 (2010). 20065073

A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation.
Hosokawa N, Wada I, Hasegawa K, Yorihuzi T, Tremblay L O, Herscovics A, Nagata K
EMBO Rep., 2, 415-422 (2001). 11375934

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Edem1ER degradation enhancer, mannosidase alpha-like 16Edem1ER degradation enhancer, mannosidase alpha-like 1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistance gene, S. cerevisiae FRT sequence, Mouse Phosphoglycerate kinase promoter
    Research application
    Specific Term and ConditionsBefore the first publication on the BIOLOGICAL RESOURCE will be published by the DEPOSITOR, the RECIPIENT of the BIOLOGICAL RESOURCE shall obtain a written consent on use of the BIOLOGICAL RESOURCE from the DEPOSITOR. After the first publication on the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR (will be announced) is requested in publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE.
    DepositorNobuko Hoshokawa (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data