Strain Data Sheet

RBRC04696

Information1

Image
BRC No.RBRC04696
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Agtr1a<tm1Afu>
Former Common nameB6-AT1a KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Akiyoshi Fukamizu, Institute of Applied Biochemistry, Tsukuba Advanced Researrch Alliance, University of Tukuba in 1995.
Strain description
Colony maintenance
ReferencesJ. Biol. Chem., 270, 18719-18722 (1995). 7642517

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Agtr1aangiotensin II receptor, type 1a13Agtr1a<tm1Afu>targeted mutation 1, Akiyoshi Fukamizu

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAMouse AT1a genomic DNA, E. coli neo<r>, E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter)[update Feb 12, 2014]
Research applicationCell Biology Research
Fluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 270, 18719-18722 (1995).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkiyoshi Fukamizu (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

Kawakami-Mori F, Nishimoto M, Reheman L, Kawarazaki W, Ayuzawa N, Ueda K, Hirohama D, Kohno D, Oba S, Shimosawa T, Marumo T, Fujita T.
Aberrant DNA methylation of hypothalamic angiotensin receptor in prenatal programmed hypertension.
JCI Insight 3(21) (2018) 30385711