RBRC04609

Information1

Image
BRC No.RBRC04609
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Wnt5a<tm1.1Homy>
Former Common nameWnt5a-floxed
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2014/07/14Room:4-ARack:J
2014/05/19Room:4-ARack:J
2014/03/24Room:4-ARack:J
2014/01/20Room:4-ARack:J
2013/11/25Room:4-ARack:J
2013/09/24Room:4-ARack:J
2013/07/22Room:4-ARack:J
2012/01/23Room:4-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Wnt5awingless-related MMTV integration site 5A14Wnt5a<tm1.1Homy>

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP14loxP

Information2

Donor DNAmouse wnt5a genomic DNA, Phage P1 loxP, yeast FRT (flippase recombination target) sites
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Abedini A, Zamberlam G, Lapointe E, Tourigny C, Boyer A, Paquet M, Hayashi K, Honda H, Kikuchi A, Price C, Boerboom D.
WNT5a is required for normal ovarian follicle development and antagonizes gonadotropin responsiveness in granulosa cells by suppressing canonical WNT signaling.
FASEB J. 30 1534-1547 (2016) 26667040